Paola Stephania/LINK TO WORKSHOP

ACTIVITIES

During the three first days, a series of activities were made;

*Day 1:

• Introduction to the laboratory working space, the location of instruments and some working techniques.

• Workshop around activities, expectations, questions around what the group wants to learn, personal experience, previous work or field of work, and knowledge that can be offered to the team.

• Presentation from Jan Glöckner, an expert in fungi work. After the presentation, a medium for fungi was prepared, a medium for one of his original samples.

Original sample of Jan Glöckner (Armillaria Gallica.)

Potatoe Dextrose Agar (PDA)

- Diced and boiled potatoes - 150g - Dextrose - 7g - In 500ml of water, boil 150g of potatoes. Strain out the solids, bring the solution back to 500ml with water, then add remaining ingredients. Five grams of potatoes flakes can be used as a substitute.

For the preparation were used: - 380 ml of diced and boiled Potatoes - 7 g of Dextrose - 7.6 g of Agar

• Alimentation and photography exercise with Hydra Vulgaris - Graver Süsswasserpolys.

*Day 2:

- Whit the Potatoe Dextrose Agar (PDA) made on the first day we cultivate/make a clone of Armillaria Gallica.

- A selected specimen Haematococcus Pluvialis is cultivated. The medium is made with 100ml of water, 3% of vinasse and 0.7% of NaCl. Note; in the laboratory was no Vinasse, so another bioproduct of the sugar was used in order to prepare the medium. Three samples were made all with the same concentration of salinity but with different percentage of vinasse, and with the same PH 7. from left to right; original medium, 10% of original medium diluted in 100ml of water, 1% of original sample diluted in 100ml of water.

- A sample of Aliivibrio Fischeri is taken from a specimen brought by a member of the group. The medium is made with 3g of NaCl, 0.1g of glycerol, 1g of Pepton, 3g of meat extract in 100ml of distilled water.

Day 3:

- The procedure of how to use the microscope, how to clean it and general rules around it correct use is taught, and also the use of the pipets and its calibration.

An observation under the microscope is made in order to see the specimen that we made the day before "Haematoccocus pluvialis"

- The group work in individual projects and start the documentation of all the information made during the first two days workshop.

- A conference around the subject Gene edition is made. General concepts are clarified in order to understand the meaning of gene edition, and a discussion around CRISPR Clustered - Regular - Interspaced - Short - Palindromic - Repeats is made.

NOTE: the following information is a short resume about the concepts that were discussed.

• • DNA: Deoxyribonucleic acid is the chemical molecule made of A-T C-G (Adenine - Thymine - Cytosine - Guanine)

• • Genetic code: the set of rules from cells.

• • Genome: all DNA from one organism.

• • Gene: organization structure (instructions - encodes a molecule)

further information:

- https://en.wikipedia.org/wiki/DNA

- https://en.wikipedia.org/wiki/Genetic_code

- https://en.wikipedia.org/wiki/Genome

- https://en.wikipedia.org/wiki/Gene

- https://en.wikipedia.org/wiki/CRISPR