Literature, Ethics, Tools: Difference between revisions

Latest revision as of 17:36, 20 March 2019

Documentation

Literature

Intro

Mycorrhizal Networks or How I Hack Plant Conversations

Ethics

Draft DIY-bio Code for ethics from European congress

Transparency. Emphasize transparency and the sharing of ideas, knowledge, data and results
Safety. Adopt safe practices
Open Access. Promote citizen science and decentralized access to biotechnology.
Education. Help educate the public about biotechnology, its benefits and implication.
Modesty. Know, you don’t know everything.
Community. Carefully listen to any concerns and questions and respond honestly.
Peaceful Purposes. Biotechnology must only be used for peaceful purposes.
Respect. Respect humans and all living systems.
Responsibility. Recognize the complexity and dynamics of living systems and our responsibility towards them.
Accountability. Remain accountable for your actions and for upholding this code.

Tools

Microscopy

Basic Microscope Setup and Use
Microscopy with Oil Immersion
- In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens. Immersion oils are transparent oils that have specific optical and viscosity characteristics necessary for use in microscopy. (https://en.wikipedia.org/wiki/Oil_immersion)
Further read and how to: http://www.micrographia.com/tutoria/micbasic/micbpt07/micb0700.htm

Microscopic Photography

- The first step is to find a subject, in my case, I want to work with Polycephalum/physarum an allowed sample in the laboratory and with samples that will be brought by my colleagues.

Types of photography with a microscope:

- Köhler Illumination: neutral and uniform backlighting, helps to maximize the contrast and resolution in the image.
  - https://www.zeiss.com/microscopy/int/solutions/reference/basic-microscopy/koehler-illumination.html
  - https://www.youtube.com/watch?v=dYP0uBxE0AA
- Darkfield: makes an object glow against a black background by using a darkfield condenser into the microscope (there are to types; dry or oil condenser).
  - https://en.wikipedia.org/wiki/Dark-field_microscopy
  - https://www.youtube.com/watch?v=Tw557w421is

DIY Microscopy

- GMU:DIY-Microscopy
- https://www.youtube.com/watch?v=9pQG9h8-nvQ

Electronic Tools

Tool Kit

[2]
[3]

References

My Collaboration with Bacteria for Paper Production [PDF]
Miya Masaoka, Pieces for Plants, http://www.youtube.com/watch?v=1AHOEcAprc8
Martin Howse, Radio Mycelium, http://libarynth.org/parn/radio_mycelium
Laura Popplow, Fungutopia, http://www.fungutopia.org/
Paul Stamets, Life Box, https://www.youtube.com/watch?v=Q8CMsB4jLLc
Donna Haraway, Anthropocene, Capitalocene, Plantationocene,

Chthulucene: Making Kin, http://environmentalhumanities.org/arch/vol6/6.7.pdf

Riedel-Kruse Lab, Biotic games: https://web.stanford.edu/group/riedel-kruse/

@@ Line 1: / Line 1: @@
-==23.10.2018 ==
+==Documentation ==
 ==='''Literature''' ===
 ====Intro====
@@ Line 19: / Line 19: @@
 ==='''Tools''' ===
 ====Microscopy====
-[[File:Immersionsvorteil.jpg|thumb|"Principle of immersion microscopy. Path of rays with immersion medium (yellow) (left half) and without (right half). Rays (black) coming from the object (red) at a certain angle and going through the coverslip (orange, as the slide at the bottom) can enter the objective (dark blue) only when immersion is used."(https://en.wikipedia.org/wiki/Oil_immersion)]]
 *[https://www.youtube.com/watch?v=SUo2fHZaZCU Basic Microscope Setup and Use]
 *Microscopy with Oil Immersion
-''In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens. Immersion oils are transparent oils that have specific optical and viscosity characteristics necessary for use in microscopy.''
+**''In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens. Immersion oils are transparent oils that have specific optical and viscosity characteristics necessary for use in microscopy.'' (https://en.wikipedia.org/wiki/Oil_immersion)
-(https://en.wikipedia.org/wiki/Oil_immersion)
 *Further read and how to: http://www.micrographia.com/tutoria/micbasic/micbpt07/micb0700.htm
 *''Microscopic Photography''
-The first step is to find a subject, in my case, I want to work with ''Polycephalum/physarum'' an allowed sample in the laboratory and with samples that will be brought by my colleagues.
+**The first step is to find a subject, in my case, I want to work with ''Polycephalum/physarum'' an allowed sample in the laboratory and with samples that will be brought by my colleagues.
 *''Types of photography with a microscope:''
-**''Köhler Illumination:'' neutral and uniform backlighting, helps to maximize the contrast and resolution in the image.
+**Köhler Illumination:'' neutral and uniform backlighting, helps to maximize the contrast and resolution in the image.
 ***https://www.zeiss.com/microscopy/int/solutions/reference/basic-microscopy/koehler-illumination.html
 ***https://www.youtube.com/watch?v=dYP0uBxE0AA
-**''Darkfield:'' makes an object glow against a black background by using a darkfield condenser into the microscope ''(there are to types; dry or oil condenser)''.
+**Darkfield:'' makes an object glow against a black background by using a darkfield condenser into the microscope ''(there are to types; dry or oil condenser).
 ***https://en.wikipedia.org/wiki/Dark-field_microscopy
 ***https://www.youtube.com/watch?v=Tw557w421is
 *''DIY Microscopy''
 **[[GMU:DIY-Microscopy]]
 **https://www.youtube.com/watch?v=9pQG9h8-nvQ
@@ Line 47: / Line 48: @@
 *[[:File:Arduino-tutorial-analog.maxpat]]
 * Arduino. https://www.arduino.cc/en/Tutorial/PWM
+**[https://www.uni-weimar.de/kunst-und-gestaltung/wiki/images/Martin-howse.pdf]
 ====Tool Kit====
-*[[:File:Mycorrhizal Plant Conversations.pdf| Mycorrhizal Networks or How I Hack Plant Conversations]]
+*[https://www.uni-weimar.de/kunst-und-gestaltung/wiki/images/Mycorrhizal_Plant_Conversations.pdf]
+*[https://www.uni-weimar.de/kunst-und-gestaltung/wiki/images/Martin-howse.pdf]
 ==='''References'''===