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*[[/ | ==My Final Project== | ||
==='''Concept'''=== | |||
This section is devoted to those non-scientists (or fledgling scientists) who are interested in simple methods for culturing Physarum. | |||
Some of the documentation included here is not easily referenced, having come from websites that are no longer available, or having been obtained via web-surfing (e.g. Google) that yielded material in one search but not another. Wherever possible I have included, in the document, the URL from which it was obtained and/or an acknowledgment of the source. I apologize for any inadvertent infringement on copyright or "intellectual property" but ask forgiveness since the sole purpose of this section is to encourage the non-expert - especially young students - to give it a try and HAVE SOME FUN. | |||
==='''Process'''=== | |||
In this part I would like to show the process of a project from the beginning to the end. | |||
*Life Cycle of Physarum polycephalum [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/GMU:DIY_Bio:_doing_things_with_biology/Niloofar_Ghanavati/Initial_Idea/Life_Cycle_of_Physarum_polycephalum] | |||
*A Simple Method of Growing the Plasmodial Slime Mold Physarum Polycephalum [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/GMU:DIY_Bio:_doing_things_with_biology/Niloofar_Ghanavati/Initial_Idea/A_Simple_Method_of_Growing_the_Plasmodial_Slime_Mold_Physarum_Polycephalum] | |||
*Growing Plasmodia [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/GMU:DIY_Bio:_doing_things_with_biology/Niloofar_Ghanavati/Developed_Idea/Growing_Plasmodia] | |||
====''Materials''==== | |||
*Petri dish (glass or plastic, need not be sterile) or other suitable container. | |||
*Paper towels (white is best, brown will work). | |||
*Old Fashioned Oatmeal Flakes (not the instant). | |||
*Tap water. | |||
*A plasmodium or sclerotium-available from biological supply houses. | |||
*Medium | |||
**100 ml of distilled water | |||
**2g of agar | |||
**oat flakes | |||
====''Methods''==== | |||
Cover container bottom with piece of paper towel. | |||
*Place piece of living plasmodium or sclerotium on paper towel. | |||
*Moisten paper towel with tap water and drain off excess water. | |||
*Add one oat flake near slime mold. | |||
*Place lid over dish and place in a dark place at room temperature (e.g. place in a drawer or closet). | |||
*Next day examine culture and sprinkle with water to give Physarum a gentle rinse and pour off excess water (rinsing improves growth). *Add 1-3 additional oat flakes near advancing front of plasmodium. | |||
*Continue to examine, rinse, and feed daily or every few days. | |||
''Additional cultures are easily made by transferring a plasmodial covered oat flake to another container. Producing several cultures is desirable to ensure that you don't loose an only culture due to a unforeseen failure of a plasmodium. Also, having more than one culture allows you to test the effects of different treatments on the slime mold (e.g. exposure to light, cold, different food sources, etc.).'' | |||
==='''Final Project'''=== | |||
Now you can see the photos of final project. | |||
<gallery> | |||
File:13.JPG|Preparation of Medium | |||
File:14.JPG|Cook Medium in Microwave | |||
File:17.JPG|Preparation of Project | |||
File:20.JPG|After 1 Day | |||
File:19.JPG|After 1 Day | |||
File:36.JPG|Documentation of mein Project | |||
File:2.JPG|After 2 Days | |||
File:51.JPG|After 3 Days | |||
File:4.JPG|After 6 Days | |||
File:49.JPG| After 8 Days | |||
File:3.JPG|After 10 Days | |||
File:5.JPG||After 15 Days | |||
File:6.JPG|After 20 Days | |||
File:7.JPG|After 25 Days | |||
File:61.JPG|After 25 Days | |||
File:8.JPG|After 30 Days | |||
File:66-1.jpg|After 45 Days | |||
File:68.JPG|The outgoing physarum from the bottle | |||
File:69.JPG|The outgoing physarum from the bottle | |||
File:70.JPG|The outgoing physarum from the bottle | |||
</gallery> | |||
==='''Refrences'''=== | |||
here are the references of a project this semester. [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/GMU:DIY_Bio:_doing_things_with_biology/Niloofar_Ghanavati/References ] |
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