GMU:DIY BioLab: Difference between revisions

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[[Marienstraße 7b]], [[Marienstraße 7b/202|Room 202]], 99423 Weimar


== Opening hours ==
Contact: <br>Mindaugas Gapševičius, mindaugas.gapsevicius [at] uni-weimar.de, <br>Alessandro Volpato, alessandro.volpato [at] uni-weimar.de <br><br>


Monday 14:00 - 17:00
== Hygieneconcept ==
ENGLISH [[:File:Hygieneconcept-DIY-Biolab-(16.11.20)_ENGLISH.pdf]]<br>
GERMAN [[:File:Hygienekonzept-DIY-Biolab-(16.11.20)_DEUTSCH.pdf]]


Wednesday 14:00 - 17:00
<b>The procedure in short</b>
* Coordination of booking on this Wiki-page (see above)
* Keys for the Biolab are handed out in the secretary's office against signature, or can be borrowed from the room managers for the time of use
* Recording of the attendance times of students via the occupancy list
* Registration of contact data via the available form
* The room must be adequately ventilated before leaving.


Friday 14:00 - 17:00


<br>


== Short introduction to our DIY Bio-Lab ==
== Short introduction to our DIY Bio-Lab ==
Line 14: Line 22:
Welcome to our DIY Bio-Lab at Bauhaus University Weimar
Welcome to our DIY Bio-Lab at Bauhaus University Weimar


Before you start using our microscopes, the laminar flow bench or chemicals I ask you kindly to take two hours to prepare beforehand.
Before you start using our microscopes, the laminar hood or chemicals, please take a couple of hours to prepare beforehand:


In these two hours, please
* book an introduction to the microscopes and understand how to use them
1. read the manuals for the microscopes and understand how to use them
* watch two videos and learn how to use microliter pipettes
2. watch two videos and learn how to use microliter pipettes
* get some basic vocabulary about lab- equipment  
3. get some basic vocabulary about lab- equipment  
* read about your organism, before you start growing it
4. read some recipes for the medium your organism needs
* research some recipes for the medium your organism needs
5. and read the basic papers about your organism, before you start growing it.


→ 1. The manuals for the lab:


das saubere Mikroskop
===Microscope===
[http://www.ehlert-partner.de/Mikroskopreinigung.pdf]  
* [http://www.ehlert-partner.de/Mikroskopreinigung.pdf das saubere Mikroskop]  
english:  
*'''The Bresser Microscope (''the big one'')''' [https://www.bresser.de/out/media/d32560c4539eb0c24bee07dbb7f2c40e.pdf Manual ]


<gallery>
File:SomeniceBacteria.png|these kind of pictures are possible with the Bresser microscope. Full rez SomeniceBacteria.JPG
File:Moldondictyostelium.png|another one of common mold spore heads. Full rez Moldondictyostelium.JPG
</gallery>


The big microscope  
* [http://www.masterflex.com/assets/Manual_pdfs/48925-30%20-35%20-40.pdf The Masterflex microscope ("the small one")]
* [http://www.kanidis.gr/common/files/MOLECULAR/EQUIPMENT/Cat_V100_i.pdf The laminar hood]


[http://archive.bresser.de/download/5770200/ANL5770200DEGB0114BRESSER.pdf]  
===microliter pipettes===
*[https://www.youtube.com/watch?v=NgosWmRjjAo Micropipetting]
*[https://www.youtube.com/watch?v=nv5Bi2Wy1KU School Pipetting 101 Common Errors]


The smaller microscope
===vocabulary===
[
*[[wikipedia:Category:Laboratory equipment|Laboratory equipment]]
http://www.masterflex.com/assets/Manual_pdfs/48925-30%20-35%20-40.pdf]
*[http://www.sciencegeek.net/Chemistry/taters/labequipment.htm Lab Equipment Quiz]
*[[wikipedia:PH|pH]]


The manual for the laminar flow bench:
===Medium===
[http://www.kanidis.gr/common/files/MOLECULAR/EQUIPMENT/Cat_V100_i.pdf]
What kind of medium you want to prepare and what your organism needs you have to find out yourself.


→ 2. The videos about the microliter pipettes:
See available [[/chemicals]] in the lab.
[https://www.youtube.com/watch?v=NgosWmRjjAo]


[https://www.youtube.com/watch?v=nv5Bi2Wy1KU]
== Current Organisms ==
*[[GMU:Habitats SS18/Physarum Polycephalum|Physarum Polycephalum]]
*[http://www.schulbiologiezentrum.info/15.20%20Euglena%20Neufassung%20031203.pdf Euglena]
*[[GMU:Experimental Microbiology/Amoeba_proteus|Amoeba proteus]]
*[[GMU:Experimental Microbiology/Armillaria_gallica|Armillaria gallica]]
*[[GMU:Experimental Microbiology/Hydra Vulgaris|Hydra_Vulgaris]]
*[[GMU:Experimental Microbiology/Haematococcus_pluvialis|Haematococcus pluvialis]]


→ 3. We work mostly in English in the DIY Biolab – so do you know what an Agar-Plate is? How much is  µl  are in 250 ml?
If you want to work with one of these organisms, please present a short concept to GMU Staff during opening hours.  
Maybe have a look at the links below and read a little about the equipment you might use. PH – value (which one does your organism want? What are the ingredients of your medium doing to the ph-value?)
If you want to work with another organism please contact us.


[http://en.wikipedia.org/wiki/Category:Laboratory_equipment]  
[http://zooundco-brehm.de/unsere-maerkte/weimar/?  Animal Store in Weimar ]  


[http://www.sciencegeek.net/Chemistry/taters/labequipment.htm]
This store has '''living''' Artemia, Daphnia, Nematocera (Chironomus & Chaoborus) and Enchytraeen ( Enchytraeus albidus). They sell them in small bags and it costs less than a coffee. Each Tuesday they will have a new delivery of these fresh organisms.
It is possible for them to get other 'fish food' as well.


[http://en.wikipedia.org/wiki/PH]  
=== Protocols ===
* [[/Euglena|Euglena]]
* [[/Amoeba|Amoeba]]
* [[/Physarum Polycephalum|Physarum Polycephalum]]
* [[/Dictyostelium|Disctyostelium]]


→ 4. + 5. What kind of medium you want to prepare and what your organism needs you have to find out yourself.
== Further Information ==
=== Research projects ===
*[[GMU:PhyChip|PhyChip]]
*[[GMU:Biogames|Biogames]]
 
=== Current Courses and Activities around Biolab ===
*[[GMU:Experimental Microbiology|Experimental Microbiology]]
*[[GMU:Bioelectronics, aesthetics and other interesting things|Bioelectronics, aesthetics and other interesting things]]
*[[GMU:Genes%26Clones|Genes&Clones]]
 
===Latest Courses===
*[[GMU:Experimental Microbiology|Experimental Microbiology]]
*[[GMU:BioArt|BioArt]]
*[[GMU:Bioart Introduction|Bioart_Introduction]]
*[[GMU:BioGames|BioGames]]
 
===Latest workshops===
*[[GMU:MediaArt and Biotechnology|MediaArt and Biotechnology]]
*[[GMU:Workshop on BioGames|Workshop on BioGames]]
*[[GMU:BioMedia Science 2 Arts- Molecular Biology entering the public space|BioMedia Science 2 Arts- Molecular Biology entering the public space]]
 
=== Links ===
*[http://www.diysect.com/ Diysect Webseries about BioArt]
 
== Photos ==
<gallery>
File:IMG_4335.png|Photo: miga
File:DSC_0197.png|Photo: Smin Kim
File:DSC_0199.png|Photo: Smin Kim
File:DSC_0203.png|Photo: Smin Kim
File:DSC_0200-1.png|Photo:miga
File:DSC_0202-1.png|Photo:miga
File:DSC_0206.png|Photo:miga
File:IMG_1368.png|Photo:miga
File:IMG_1371.png|Photo:miga
</gallery>

Latest revision as of 22:40, 24 June 2023

Marienstraße 7b, Room 202, 99423 Weimar

Contact:
Mindaugas Gapševičius, mindaugas.gapsevicius [at] uni-weimar.de,
Alessandro Volpato, alessandro.volpato [at] uni-weimar.de

Hygieneconcept

ENGLISH File:Hygieneconcept-DIY-Biolab-(16.11.20)_ENGLISH.pdf
GERMAN File:Hygienekonzept-DIY-Biolab-(16.11.20)_DEUTSCH.pdf

The procedure in short

  • Coordination of booking on this Wiki-page (see above)
  • Keys for the Biolab are handed out in the secretary's office against signature, or can be borrowed from the room managers for the time of use
  • Recording of the attendance times of students via the occupancy list
  • Registration of contact data via the available form
  • The room must be adequately ventilated before leaving.



Short introduction to our DIY Bio-Lab

Welcome to our DIY Bio-Lab at Bauhaus University Weimar

Before you start using our microscopes, the laminar hood or chemicals, please take a couple of hours to prepare beforehand:

  • book an introduction to the microscopes and understand how to use them
  • watch two videos and learn how to use microliter pipettes
  • get some basic vocabulary about lab- equipment
  • read about your organism, before you start growing it
  • research some recipes for the medium your organism needs


Microscope

microliter pipettes

vocabulary

Medium

What kind of medium you want to prepare and what your organism needs you have to find out yourself.

See available /chemicals in the lab.

Current Organisms

If you want to work with one of these organisms, please present a short concept to GMU Staff during opening hours. If you want to work with another organism please contact us.

Animal Store in Weimar

This store has living Artemia, Daphnia, Nematocera (Chironomus & Chaoborus) and Enchytraeen ( Enchytraeus albidus). They sell them in small bags and it costs less than a coffee. Each Tuesday they will have a new delivery of these fresh organisms. It is possible for them to get other 'fish food' as well.

Protocols

Further Information

Research projects

Current Courses and Activities around Biolab

Latest Courses

Latest workshops

Links

Photos