GMU:DIY Biolab “Driver’s License”/Jiannan Zhang: Difference between revisions

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'''29/11/2017----Reference to movie "Life"'''


Experimenting with one cellular organism, slime mold, physarum polycefalum
== ''''Brainless' but 'intelligent' slime mold''' ==


Similarity to human brain


to grow slime mold on different glasses in order to achieve 3d model
[[/1.Project introduction]]


Maybe is better to start with simulation in order make needed paterns
[[/2.Research methods]]


Next stage would be with living organism: at the point when it is grown, to dry it
[[/3.Experiment]]


[[:File:biolab-idea-jiannan-pdf.pdf]]
[[/4.Conclusion]]


 
[[/5.References]]
 
== '''1.Experiment''' ==
 
 
'''15/12/2017---experiment 1: start with waking up the slime molds'''
 
To have better samples to build the art installation, try to wake up old slime molds samples by growing them in the medium.
 
four samples:
 
A-normal medium(pure agar, salt, yeast, water..)+8-10 pieces of cereals
 
B-normal medium
 
C&D-normal medium+wet and old filter paper
 
samples are kept in dark lockers under 20 degrees
[[File:15:12-1.jpg|400px]][[File:15:12-2.jpg|400px]][[File:15:12-2.jpg|400px]][[File:15:12-3.jpg|400px]][[File:15:12-4.jpg|400px]]
 
'''20/12/2017---experiment 2: waking up new slime molds samples from Sinan'''
 
samples from the last experiment failed.
 
Sample A was seriously contaminated. Nothing happened in sample B, C, &D
 
reason could be too much cereals or others
 
repeat the experiment with 3 more samples:
 
all in same condition: medium(Compound agar, water...)+1 piece of cereal
 
samples are kept in dark lockers under 24-26 degrees
 
 
'''21/12/2017---Update on experiment 2: bacteria'''
 
the samples look like contaminated by some bacteria in the middle
 
 
'''23/12/2017---Update on experiment 2: failed'''
 
the samples are contaminated and slime molds didn't wake up
 
reasons could be the temperature was too warm for the slime molds, food was too little and it was not humid enough inside the mediums
 
 
 
'''02/01/2018---experiment 3: grow new samples(from sinan) under suitable temperature'''
 
repeat the experiment with 4 more samples:
 
all in same condition: medium(Compound agar, water...)+3 pieces of cereal
 
A-sealed and kept on a shelf, 20 degrees;
 
B-not sealed and in a locker, 22 degrees;
 
C-sealed and kept in a locker, 22 degrees;
 
D-not sealed and kept on a shelf, 20 degrees.
 
 
'''08/01/2018---Update on experiment 3: possibly slime molds once woke up but died afterwards'''
 
all the samples are contaminated to some extend, but sample B shows similar trace of growing slime molds according to the shape
 
I will try this sample again and another ones with new humid filter papers and wood chips(which i searched online are good mediums for slime molds)
 
got the sections of the human brain model, 10 layers in total

Latest revision as of 20:49, 30 January 2018