GMU:DIY Biolab “Driver’s License”/Eva Walch: Difference between revisions

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In this class I was researching, how bacteria grow. How they differ, depending on the media.
In this class I was researching, how bacteria grow. How they differ, depending on the media.
I  also tried to combine paintings with bacteria
I  also tried to combine drawings with bacteria




 
===''' Painting with bacteria on paper'''===
 
==='''FIRST TRY: Painting with bacteria on paper'''===


In this first try I started with cooking the standart medium out of Miga`s protocol. I also started with figuring out how the medium and bacteria are behaving on paper. Therefor I was cluing the some drawings on some stable underground, to prevent them from rolling, when they are getting wet
In this first try I started with cooking the standart medium out of Miga`s protocol. I also started with figuring out how the medium and bacteria are behaving on paper. Therefor I was cluing the some drawings on some stable underground, to prevent them from rolling, when they are getting wet




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: Distilled water – 500 ml  
: Distilled water – 500 ml  
: Agar – 7.5 g
: Agar – 7.5 g


<gallery>
<gallery>
Line 29: Line 25:
</gallery>
</gallery>


after three day you can see, that the bacteria are growing more on the paper than on the medium, there is maybe no need for the agar in the medium because maybe the nutrients were soaked into the paper. (--> see second try without agar)


After three day you can see, that the bacteria are growing more on the paper than on the medium, there is maybe no need for the agar in the medium because maybe the nutrients were soaked into the paper. (--> see second try without agar)




Line 41: Line 37:




So I went on with painting on medium and started to figure out how I can control the bacteria (see experiment further down)
But still I tried to une the same medium but without agar
 
<gallery>
File:MediumOnPAper.jpg
File:WaterResult.jpg
</gallery>
 
 
It looks like there is probably not enough liquid, so it did not really work without agar and I went on with the standard medium and started to figure out how I can control the bacteria (see experiment further below)


<gallery>
<gallery>
Line 50: Line 54:


When I had a little more knowledge I also tried to let grow two extracted bacteria on two different parts on the drawing. I used some darker, blue bacteria for the mushroom (1) and some green/white looking ones for the surrounding (2).
When I had a little more knowledge I also tried to let grow two extracted bacteria on two different parts on the drawing. I used some darker, blue bacteria for the mushroom (1) and some green/white looking ones for the surrounding (2).
But sadly it did not really work Maybe. it would be more successful to not only use two different kind of bacteria, because they somehow also could move to and other parts of the picture just through the air, but also use two different kind of media.
But sadly it did not really work. Maybe it would be more successful if I do not only use two different kind of bacteria, because they somehow also could move to and other parts of the picture just through the air, but also use two different kind of media.
   
   
<gallery>
<gallery>
File:PilzAnmalen.jpg|400px
File:PilzAnmalen.jpg
File:GemalterPilz.jpg|400px
File:GemalterPilz.jpg
</gallery>
</gallery>


:  --> it did not really work, maybe I also have to use different kind of media, for not letting the bacteria mix on the paper
==='''SECOND TRY:'''===
• Cooking the same medium but without agar
[[File:Medium.jpg|400px]]
• Just putting the medium on some paper to see what happens without agar
[[File:MediumOnPAper.jpg|400px]]
• After 2.5 weeks:
[[File:WaterResult.jpg|400px]]
:  -->There was probably not enough liquid, so it did not really work without agar




'''Making different kind of media to create different kind of bacteria fields :'''
==='''Media experiments:'''===


one way could be to change the PH value (source: Wikipedia [https://de.wikipedia.org/wiki/Selektivnährboden]):
In these tries, I was starting to figure out how I can let grow diffenent kind of bacteria.
One way is to change the ph-level of the medium
(source: Wikipedia [https://de.wikipedia.org/wiki/Selektivnährboden]):


"Unter Selektivnährboden versteht man einen Nährboden, der das Wachstum einer bestimmten Gruppe von Mikroorganismen, beispielsweise bestimmter Bakterien, begünstigt während er die Entwicklung anderer Mikroorganismen unterdrückt. Dies geschieht aufgrund der Zusammensetzung des Nährbodens, da Mikroorganismen unterschiedliche Bedürfnisse hinsichtlich des Kulturmediums in puncto Nährstoffzusammensetzung, '''pH-Wert''' etc. besitzen und da einige Stoffe andere Mikroorganismen in ihrem Wachstum hemmen."
"Unter Selektivnährboden versteht man einen Nährboden, der das Wachstum einer bestimmten Gruppe von Mikroorganismen, beispielsweise bestimmter Bakterien, begünstigt während er die Entwicklung anderer Mikroorganismen unterdrückt. Dies geschieht aufgrund der Zusammensetzung des Nährbodens, da Mikroorganismen unterschiedliche Bedürfnisse hinsichtlich des Kulturmediums in puncto Nährstoffzusammensetzung, '''pH-Wert''' etc. besitzen und da einige Stoffe andere Mikroorganismen in ihrem Wachstum hemmen."


I tried to cook media with different kind of acidity
Here I tried to cook media with different kind of acidity
#in the first petri dish there is the medium for the Growth of Gluconacetobacter but without citric acid
#in the first petri dish there is the medium for the Growth of Gluconacetobacter but without citric acid
#in the second petri dish there is the standart medium for the growth of Gluconacetobacter, which includes some citric acid
#in the second petri dish there is the standart medium for the growth of Gluconacetobacter, which includes some citric acid
#the third petri dish contains the medium for the growth of Gluconacetobacter, but with the double the amound of citric acid
#the third petri dish contains the medium for the growth of Gluconacetobacter, but with the double the amound of citric acid


<gallery>
File:VerschiedeneMedien.jpg
File:Nährböden.jpg
File:Nährböden2.jpg
</gallery>


[[File:VerschiedeneMedien.jpg|400px]]
[[File:Nährböden.jpg|400px]]
[[File:Nährböden2.jpg|400px]]


So I found out, that with to less acidity there is nothing really happening and with more acidity than usual there are no more bacteria growing, but fungi.
What I also realized, is that you can play with the different kind of sugar you are using to cook the medium.
It makes a big different, if you use glucose or fructose, but also you can use for example potato starch.


'''Coloring bacteria:'''
<gallery>
File:FructoseMedium.jpg
</gallery>


In this experiment I try to color bacteria after they grown


Furthermore I was trying to extract two different bacteria into two different petri dishes to have to clean bacteria to be able to paint with them


• just cooked media for the Growth of Gluconacetobacter, but used Fructose instead of Glucose
<gallery>
File:extractedBacteria.jpg
File:extractedBacteria2.jpg
File:extractedBacteria3.jpg
</gallery>




after 1 week


[[File:FructoseMedium.jpg|400px]]
==='''Coloring bacteria and media:'''===




trying to extract two different bacteria into two different petri dishes
In this experiment I try to color bacteria after they grown, by putting red pigment to the bacteria


[[File:extractedBacteria.jpg|400px]]
<gallery>
[[File:extractedBacteria2.jpg|400px]]
File:RotePigmente.jpg
[[File:extractedBacteria3.jpg|400px]]
</gallery>




putting red pigment to the bacteria
After one week:


[[File:RotePigmente.jpg|400px]]
<gallery>
File:Rot1.jpg
File:Rot2.jpg
</gallery>


So how you can see, the bacteria does not absorb the color. That's probably because there are two different kind of bacteria, one whose cell wall allows it to absorb color, and the other one doesn`t.


after one week:


[[File:Rot1.jpg|400px]]
I also tried to put ink into the medium, so that it became blue. But after heating it up in the microwave the blue color disappeared.
[[File:Rot2.jpg|400px]]
But still the bacteria were growing different than on the normal medium, somehow more intense, maybe because there is more bakteria inside with the added ink


<gallery>
File:InkMedium.jpg
</gallery>




'''PUTTING COLOR INTO THE MEDIUM'''
In the second try, I put ink into the medium, but after the heating in the microwave


 
<gallery>
in this try I was putting ink into the medium, so that it became blue. But after heating it up in the microwave the blue color disappeared.
File:ink.jpg
But still the bacteria were growing different than on the normal medium, somehow more intense, maybe because there is more bakteria inside with the added ink
File:ink2.jpg
 
File:ink3.jpg
[[File:InkMedium.jpg|400px]]
</gallery>




in the second try, I put ink into the medium, but after the heating in the microwave
It looks like that the bacteria is growing much faster on the part of the medium, where there is no ink. But also where the ink is there are growing bacteria.
It also seems like they are soaking in the color.


[[File:ink.jpg|400px]]
[[File:ink2.jpg|400px]]
[[File:ink3.jpg|400px]]




Further steps:
===Further steps:===


#making the bacteria on the paper permanent, with maybe lack
Now it would be interesting to let grow different bacteria in the same petri dish. Therefore I would use different kind of media, that means using different kind of sugar, and also playing with the acidity.
#using different kind of media to create different kind of bacteria fields
Furthermore I could choose between the extracted bacteria, depending on what kind of bacteria I want to have on which part of the petri dish.
#creating pictures with more controled media
#putting color into the medium
#putting different kind of media in the same petri dish or on one picture

Latest revision as of 22:20, 14 February 2018

BACTERIA INTERACTION WITH PAINTING

In this class I was researching, how bacteria grow. How they differ, depending on the media. I also tried to combine drawings with bacteria


Painting with bacteria on paper

In this first try I started with cooking the standart medium out of Miga`s protocol. I also started with figuring out how the medium and bacteria are behaving on paper. Therefor I was cluing the some drawings on some stable underground, to prevent them from rolling, when they are getting wet


Medium recipe (source: Miga’s protocol)

Glucose – 10 g
Peptone – 2.5 g
Yeast extract – 2.5 g
Na2HPO4 – 1.35 g
Citric acid – 0.75 g
Distilled water – 500 ml
Agar – 7.5 g


After three day you can see, that the bacteria are growing more on the paper than on the medium, there is maybe no need for the agar in the medium because maybe the nutrients were soaked into the paper. (--> see second try without agar)


After 2,5 weeks it changed and the bacteria were growing strong on the medium


But still I tried to une the same medium but without agar


It looks like there is probably not enough liquid, so it did not really work without agar and I went on with the standard medium and started to figure out how I can control the bacteria (see experiment further below)


When I had a little more knowledge I also tried to let grow two extracted bacteria on two different parts on the drawing. I used some darker, blue bacteria for the mushroom (1) and some green/white looking ones for the surrounding (2). But sadly it did not really work. Maybe it would be more successful if I do not only use two different kind of bacteria, because they somehow also could move to and other parts of the picture just through the air, but also use two different kind of media.


Media experiments:

In these tries, I was starting to figure out how I can let grow diffenent kind of bacteria. One way is to change the ph-level of the medium (source: Wikipedia [1]):

"Unter Selektivnährboden versteht man einen Nährboden, der das Wachstum einer bestimmten Gruppe von Mikroorganismen, beispielsweise bestimmter Bakterien, begünstigt während er die Entwicklung anderer Mikroorganismen unterdrückt. Dies geschieht aufgrund der Zusammensetzung des Nährbodens, da Mikroorganismen unterschiedliche Bedürfnisse hinsichtlich des Kulturmediums in puncto Nährstoffzusammensetzung, pH-Wert etc. besitzen und da einige Stoffe andere Mikroorganismen in ihrem Wachstum hemmen."

Here I tried to cook media with different kind of acidity

  1. in the first petri dish there is the medium for the Growth of Gluconacetobacter but without citric acid
  2. in the second petri dish there is the standart medium for the growth of Gluconacetobacter, which includes some citric acid
  3. the third petri dish contains the medium for the growth of Gluconacetobacter, but with the double the amound of citric acid


So I found out, that with to less acidity there is nothing really happening and with more acidity than usual there are no more bacteria growing, but fungi. What I also realized, is that you can play with the different kind of sugar you are using to cook the medium. It makes a big different, if you use glucose or fructose, but also you can use for example potato starch.


Furthermore I was trying to extract two different bacteria into two different petri dishes to have to clean bacteria to be able to paint with them


Coloring bacteria and media:

In this experiment I try to color bacteria after they grown, by putting red pigment to the bacteria


After one week:

So how you can see, the bacteria does not absorb the color. That's probably because there are two different kind of bacteria, one whose cell wall allows it to absorb color, and the other one doesn`t.


I also tried to put ink into the medium, so that it became blue. But after heating it up in the microwave the blue color disappeared. But still the bacteria were growing different than on the normal medium, somehow more intense, maybe because there is more bakteria inside with the added ink


In the second try, I put ink into the medium, but after the heating in the microwave


It looks like that the bacteria is growing much faster on the part of the medium, where there is no ink. But also where the ink is there are growing bacteria. It also seems like they are soaking in the color.


Further steps:

Now it would be interesting to let grow different bacteria in the same petri dish. Therefore I would use different kind of media, that means using different kind of sugar, and also playing with the acidity. Furthermore I could choose between the extracted bacteria, depending on what kind of bacteria I want to have on which part of the petri dish.