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Those so called swarm assays or swarm agar plates are really simple to prepare, easy to use. If you used different bacteria expressing color pigments (red, green, black), I am sure you could see how one bacteria would take over the space of the others, mix up with them, kill them etc. | Those so called swarm assays or swarm agar plates are really simple to prepare, easy to use. If you used different bacteria expressing color pigments (red, green, black), I am sure you could see how one bacteria would take over the space of the others, mix up with them, kill them etc. | ||
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'''''b) making bacteria kill other bacteria''''' | '''''b) making bacteria kill other bacteria'''''<br/> | ||
In 2008 we also developed a killing mechanism which worked pretty good. The principle is the following: One bacterial strain was engineered to secrete a certain messenger molecule, called Autoinducer-1. The other bacterial strain was engineered to recognize Autoinducer-1 and thereby activate the production of a bacterial toxin called colicin [http://2008.igem.org/Team:Heidelberg/Project/Killing_II]. This means: If both bacterial strains are nearby each other, the one would start to kill the other. It is no problem to label one bacterial strain in red and the other in green and to visualize how the green bacteria die if they are nearby the red ones (figure 17 again on [http://2008.igem.org/Team:Heidelberg/Project/Killing_II this] page). The same should also be possible on agar | In 2008 we also developed a killing mechanism which worked pretty good. The principle is the following: One bacterial strain was engineered to secrete a certain messenger molecule, called Autoinducer-1. The other bacterial strain was engineered to recognize Autoinducer-1 and thereby activate the production of a bacterial toxin called colicin [http://2008.igem.org/Team:Heidelberg/Project/Killing_II]. This means: If both bacterial strains are nearby each other, the one would start to kill the other. It is no problem to label one bacterial strain in red and the other in green and to visualize how the green bacteria die if they are nearby the red ones under a microscope (figure 17 again on [http://2008.igem.org/Team:Heidelberg/Project/Killing_II this] page). The same should also be possible on swarm agar plates. | ||
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With swimming bacteria on swarm agar plates, you have many options to play around, as their are so many different bacterial strains (swimming slower, faster, not at all, recognizing substances etc.). Take this just as some general comments to think about. In case their is something in that comments, that interests you in particular, please don't hesitate to shoot me an e-mail. As I did my bachelor thesis on this topic (swimming bacteria), I would be very happy to provide all necessary information and help. | With swimming bacteria on swarm agar plates, you have many options to play around, as their are so many different bacterial strains (swimming slower, faster, not at all, recognizing substances etc.). Take this just as some general comments to think about. In case their is something in that comments, that interests you in particular, please don't hesitate to shoot me an e-mail. As I did my bachelor thesis on this topic (swimming bacteria), I would be very happy to provide all necessary information and help. | ||