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==== '''Day 2 Preparation of Grain Spawn''' ==== | ==== '''Day 2 Preparation of Grain Spawn''' ==== | ||
Objective: Prepare grain substrate in a jar to produce biomass. | |||
Materials: pressure cooker, scale, distilled water, rice grains, gypsum (CaSO4 - calcium sulphate), spoon, plate with well-growing mycelium, scalpel, gloves, ethanol, jar, wadding | |||
<u>Step 1: Weigh Materials</u> | |||
{| class="wikitable" | |||
|+ | |||
!Component | |||
!Quantity | |||
! | |||
!Component Quantity Custom Quantity | |||
|- | |||
|rice grain | |||
|30 | |||
|multiplier | |||
|g | |||
|- | |||
|Calcium sulphate (gypsum) | |||
|(2%) 0.6g | |||
|multiplier | |||
|g | |||
|} | |||
<u>Step 2: Soak rice Grains and Add Gypsum</u> | |||
# Pour boiling distilled water over rice grains and allow them to soak for a minimum of one hour to absorb the water fully. | |||
# Drain any excess liquid. | |||
# Combine the gypsum with the soaked rice grains, ensuring thorough mixing. | |||
# Transfer the mixture into a heat-resistant glass jar. | |||
# Create a ventilation hole in the lid and insert cotton wadding for mushroom air exchange. | |||
# Seal the jars and cover the lid with aluminum foil. | |||
<gallery> | |||
File:Mixed rice 2.jpg | |||
File:Mixed rice 3.jpg | |||
File:Mixed rice.jpg | |||
</gallery><u>Step 3: Sterilize In Pressure Cooker</u> | |||
<u>Step 4: Add Mycelium</u> | |||
<u>Step 5: Incubate</u> | |||
For reference to the original experiment, please see [https://www.top-ev.de/wp-content/uploads/2021/12/Prepare-rye-jars-v2_1-1.pdf Preparation of Grain Spawn] | For reference to the original experiment, please see [https://www.top-ev.de/wp-content/uploads/2021/12/Prepare-rye-jars-v2_1-1.pdf Preparation of Grain Spawn] | ||