GMU talk:Synthetic Biology/Bacteria Game/iGEM Wiki: Difference between revisions
Discussion page of GMU:Synthetic Biology/Bacteria Game/iGEM Wiki
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== Notebook == | == Notebook == | ||
=== 29/10/2010 === | === 29/10/2010 === | ||
* [http://2010.igem.org/Transformation transformation] of TOP10 cells with plasmids out of the registry following [http://partsregistry.org/Help:Spring_2010_DNA_distribution#DNA_Kit_Plate_Instructions standard recommendations] | * [http://2010.igem.org/Transformation transformation] of TOP10 cells with plasmids (see table 1) out of the registry following [http://partsregistry.org/Help:Spring_2010_DNA_distribution#DNA_Kit_Plate_Instructions standard recommendations] | ||
* over-night growth on selective agar plates | * over-night growth on selective agar plates | ||
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{| class="wikitable sortable" , border="1" | {| class="wikitable sortable" , border="1" | ||
|- bgcolor=grey | |- bgcolor=grey | ||
! height=20px, align='center', width=80px | plasmid|| width=150px | Part || width=150px | pigment color || width=150 px | backbone || width= | ! height=20px, align='center', width=80px | plasmid|| width=150px | Part || width=150px | pigment color || width=150 px | backbone || width=150 | registry location | ||
|-style="height:20px" | |-style="height:20px" | ||
|+ align="bottom" |''<b>table 1:</b>'' overview of used iGEM constructs | |+ align="bottom" |''<b>table 1:</b>'' overview of used iGEM constructs | ||
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* glycerol stocks (prepared directly after strains were received) from predator and prey cells (see table 2) were plated on selective agar plates | |||
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! height=20px, align='center', width=80px | sample || width=150px | name/function || width=150px | cell strain || width=350 px | plasmid || width=100 | marker | ! height=20px, align='center', width=80px | sample || width=150px | name/function || width=150px | cell strain || width=350 px | plasmid || width=100 | marker | ||
|-style="height:20px" | |-style="height:20px" | ||
|+ align="bottom" |''table | |+ align="bottom" |''table 2:'' used predator-prey system, gift from R. Smith | ||
|-align="center" | |-align="center" | ||
| style="font-weight:bold;" | 1a || predator || MG1655 || ptetLuxRLasI-luxCcdA(SC101), placCcdBs-tetGFPuv(LVA) || Cm, Kan | | style="font-weight:bold;" | 1a || predator || MG1655 || ptetLuxRLasI-luxCcdA(SC101), placCcdBs-tetGFPuv(LVA) || Cm, Kan | ||