GMU talk:Synthetic Biology/Bacteria Game/iGEM Wiki: Difference between revisions
Discussion page of GMU:Synthetic Biology/Bacteria Game/iGEM Wiki
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The showdown competition is run by synthetic bacteria. Predators and prey communicate and regulate each other's density. Via molecular signals, the predator cells kill the prey while living prey rescues predators. The diverse and colorful crowd surrounding the spectacle was genetically engineered to carry different pigments which has been appreciated at the iGEM competition 2009. | The showdown competition is run by synthetic bacteria. Predators and prey communicate and regulate each other's density. Via molecular signals, the predator cells kill the prey while living prey rescues predators. The diverse and colorful crowd surrounding the spectacle was genetically engineered to carry different pigments which has been appreciated at the iGEM competition 2009. | ||
== Notebook == | == Wetlab Notebook == | ||
* usage of [http://2009.igem.org/Team:Cambridge E. chromi principle] (pigmented ''E. coli'' cells) | * usage of [http://2009.igem.org/Team:Cambridge E. chromi principle] (pigmented ''E. coli'' cells) | ||
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* testing of a synthetic ''E.coli'' predator-prey system ([http://www.nature.com/nchembio/journal/v5/n12/pdf/nchembio.244.pdf Song ''et al.'', 2009]), ([http://www.nature.com/msb/journal/v4/n1/pdf/msb200824.pdf Ballagaddé ''et al.'', 2008]) | * testing of a synthetic ''E.coli'' predator-prey system ([http://www.nature.com/nchembio/journal/v5/n12/pdf/nchembio.244.pdf Song ''et al.'', 2009]), ([http://www.nature.com/msb/journal/v4/n1/pdf/msb200824.pdf Ballagaddé ''et al.'', 2008]) | ||
** for final assault on the swarming plate and under the microscope | ** for final assault on the swarming plate and under the microscope | ||
*** kindly provided by R. Smith | *** kindly provided by R. Smith (see table 2) | ||
* wildtype ''E. coli'' | * wildtype ''E. coli'' | ||
** for the game-kit | ** for the game-kit | ||
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** incubation of swarm plates at room temperature for 20 h | ** incubation of swarm plates at room temperature for 20 h | ||
*** sufficient humidity was ensured by petri dishes filled with water | *** sufficient humidity was ensured by petri dishes filled with water | ||
** photo was taken (Canon EOS 5D Mark II) every 30 sec. for 6 h | |||
* inoculation of over-night cultures from the day cultures | * inoculation of over-night cultures from the day cultures | ||
=== 01/10/2010 === | === 01/10/2010 === | ||
* preparation and conduction of microscopy experiments with predator-prey system following [http://www.nature.com/nchembio/journal/v5/n12/extref/nchembio.244-S1.pdf liquid-phase protocols] | * preparation and conduction of microscopy experiments with predator-prey system following [http://www.nature.com/nchembio/journal/v5/n12/extref/nchembio.244-S1.pdf liquid-phase protocols] | ||
** snapshots were taken every second for one minute at beginning and at the very end of the experiments | |||
** time-lapse took 3 h with a picture each 20 seconds using given filters for GFPuv and mCherry | |||