GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata: Difference between revisions
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After a while of research, I decided to focus on isolating distinct types of bacteria with the aim showing their inter-communication regarding food, as well as their dyeing abilities. Indeed, this idea is not electronics-related, but the good news is, that the name of this course has also the “aesthetics and other interesting stuff” part on it. | After a while of research, I decided to focus on isolating distinct types of bacteria with the aim showing their inter-communication regarding food, as well as their dyeing abilities. Indeed, this idea is not electronics-related, but the good news is, that the name of this course has also the “aesthetics and other interesting stuff” part on it. | ||
= A visual representation of bacterial communication systems = | |||
==Streptomyces isolation from pure soil== | |||
I. Air dry the soil samples for '''3-4 h at 45°''' to select for Actinomyces spores, considering that many other bacteria die during this pre-treatment | I. Air dry the soil samples for '''3-4 h at 45°''' to select for Actinomyces spores, considering that many other bacteria die during this pre-treatment | ||
II. Plate the samples either on to '''Actinomycete Isolation Agar (AIA) or Mannitol Soy Agar (MSA)''' | II. Plate the samples either on to '''Actinomycete Isolation Agar (AIA) or Mannitol Soy Flour Agar (MSA)''' | ||
III. Incubate at '''30°C for 7 days''' | III. Incubate at '''30°C for 7 days''' | ||
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Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria | Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria | ||
''Recipe: Mannitol Soy Flour Agar | |||
'' | |||
[[File:Mannitol.jpg|thumb]] | |||
# Considering the pattern-forming abilities of ''Paenibacillus Vortex'', I am interested in depicting the communication pathways between nutrients and bacteria. Basically, how the bacteria reach for its nutrients. I am planning on isolating the ''Paenibacillus Vortex'' on agar and adding ''Pepton'' as a nutrient. | # Considering the pattern-forming abilities of ''Paenibacillus Vortex'', I am interested in depicting the communication pathways between nutrients and bacteria. Basically, how the bacteria reach for its nutrients. I am planning on isolating the ''Paenibacillus Vortex'' on agar and adding ''Pepton'' as a nutrient. | ||