GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata: Difference between revisions

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I. Air dry the soil samples for '''3-4 h at 45°''' to select for Actinomyces spores, considering that many other bacteria die during this pre-treatment  
I. Air dry the soil samples for '''3-4 h at 45°''' to select for Actinomyces spores, considering that many other bacteria die during this pre-treatment  


II. Plate the samples either on to '''Actinomycete Isolation Agar (AIA) or Mannitol Soy Flour Agar (MSA)'''  
II. Plate the samples either on to '''Mannitol Soy Flour Agar (MSA)'''  


''Necessities for the recipe of Mannitol Soy Flour Agar''
''Necessities for the recipe of Mannitol Soy Flour Agar''
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* 10 mM CaCl2
* 10 mM CaCl2
* 100 mL Deionised water
* 100 mL Deionised water


III. Incubate at '''30°C for 7 days'''  
III. Incubate at '''30°C for 7 days'''  
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Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria


====2nd step: Adding beef extract====
====2nd step: Adding nutritive elements====


The beef extract is added in order to identify the possible communication pathways of Streptomyces when it comes to nutrition.  
A variety of nutritive elements, such as ''sugar, oats, yeast'' etc. is added in order to identify the selective abilities and possible communication pathways of Streptomyces when it comes to nutrition.  


===PAENIBACILLUS VORTEX===
===PAENIBACILLUS VORTEX===


[[Image:Vortexx.png|250px]]
[[Image:Vortexx.png|250px]]
# Considering the pattern-forming abilities of ''Paenibacillus Vortex'', I am interested in depicting the possible networks this bacterium can build through quorum sensing, when it comes to the presence of nutrition. Known as one of the most intelligent bacterium species due to its advanced quorum sensing, PV might as well be the proper organism to give clear and differential communication structures.
===1st step: Medium prep===


''Currently waiting for the bacterial strain to get delivered from Jena''
In a 250mL conical flask mix:
*3g agar
*4g peptone
*200 mL distilled water


# Considering the pattern-forming abilities of ''Paenibacillus Vortex'', I am interested in depicting the possible networks this bacterium can build through quorum sensing, when it comes to the presence of nutrition. Known as one of the most intelligent bacterium species due to its advanced quorum sensing, PV might as well be the proper organism to give clear and differential communication structures.  
[[Image:PVagar.png|200px|thumb|right|Nutritive Agar]]


====1st step: Isolation and incubation====
====1st step: Isolation and incubation====


====2nd step: Adding Pepton====
====2nd step: Adding Pepton====
''The bacterial strain of Paenibacillus Vortex was obtained from the Microbiology Research Institute of the Friedrich-Schiller University in Jena.''


'''Inspiration''':
'''Inspiration''':
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