GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata: Difference between revisions
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:'''4th step''': Prepare the Mannitol Soy Agar (MSA) petri dishes | :'''4th step''': Prepare the Mannitol Soy Agar (MSA) petri dishes | ||
<pre> | |||
MSA RECIPE: | |||
In a 250 mL conical flask mix: | |||
2g Agar | |||
In a | 2g Mannitol | ||
2g Soya flour (Holland and Barrett) | |||
10 mM CaCl2 | |||
100 mL Deionised water | |||
Medium alternative: Inorganic Salt Starch Agar (ISSA) | Medium alternative: Inorganic Salt Starch Agar (ISSA) | ||
</pre> | |||
:'''5th step''': Plate an aliquot of ''0.1ml of each dilution'' on the medium (MSA) | :'''5th step''': Plate an aliquot of ''0.1ml of each dilution'' on the medium (MSA) | ||