GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata: Difference between revisions
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:'''2nd step''':Dilute ''1-3g of soil in 10 ml saline solution'' (NaCl 0,9%) and let the solution stand for ''15 min'' | :'''2nd step''':Dilute ''1-3g of soil in 10 ml saline solution'' (NaCl 0,9%) and let the solution stand for ''15 min'' | ||
====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it==== | |||
====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes==== | |||
<pre> | <pre> | ||
MSA RECIPE: | MSA RECIPE: | ||
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</pre> | </pre> | ||
====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)==== | |||
====6th step: Incubate at 30° for 5-7 days==== | |||
::Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria | |||
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