No edit summary |
No edit summary |
||
| Line 23: | Line 23: | ||
So i took put on my new rollerskates (3) and desinfected the whole kitchen with Korn (4), a typical thuringian alcohol. Then I boiled the coffee mold (5) for 15 minutes, maybe longer and put it in a bowl to let it cool down (6). Then i desinfected an old paprika sauce glass with Korn and put the boiled mold inside (7) rotatory with pieces of the dropped spores (8&9-11) and tapped it with kitchen paper. In the end i heated my oven to 50 degrees, let it cool down and then stored the glass in it with circulating air (12). Later that day i went to the lab to put in in the real machine. | So i took put on my new rollerskates (3) and desinfected the whole kitchen with Korn (4), a typical thuringian alcohol. Then I boiled the coffee mold (5) for 15 minutes, maybe longer and put it in a bowl to let it cool down (6). Then i desinfected an old paprika sauce glass with Korn and put the boiled mold inside (7) rotatory with pieces of the dropped spores (8&9-11) and tapped it with kitchen paper. In the end i heated my oven to 50 degrees, let it cool down and then stored the glass in it with circulating air (12). Later that day i went to the lab to put in in the real machine. | ||
<gallery> | |||
File:PA310847.JPG | |||
File:PA310850.JPG | |||
File:PA310848.JPG | |||
File:PA310855.JPG | |||
File:PA310851.JPG | |||
File:PA310866.JPG | |||
File:PA310867.JPG | |||
File:PA310870.JPG | |||
File:PA310873.JPG | |||
File:PA310874.JPG | |||
</gallery> | |||
| Line 49: | Line 50: | ||
Only the sequence between the beginning and end primer is being redoubled, that makes diffrent DNA´s comparable. At least parts of it. I think Julian brought us the primer that is used to seperate the part of the Helix that stores the information about our regional origin. | Only the sequence between the beginning and end primer is being redoubled, that makes diffrent DNA´s comparable. At least parts of it. I think Julian brought us the primer that is used to seperate the part of the Helix that stores the information about our regional origin. | ||
<gallery> | |||
File:PB080907.JPG | |||
File:PB080925.JPG | |||
File:PB080932.JPG | |||
</gallery> | |||
7. Day. Today we wanted to use gel electrophoresis to compare and analize our DNA, but it didn´t work. We don´t know why. | 7. Day. Today we wanted to use gel electrophoresis to compare and analize our DNA, but it didn´t work. We don´t know why. | ||
| Line 78: | Line 79: | ||
I didn´t want to throw away the rest of the culture, so i just cut out the contamination, to see if the mycelium survives or can take back the control. | I didn´t want to throw away the rest of the culture, so i just cut out the contamination, to see if the mycelium survives or can take back the control. | ||
<gallery> | |||
File:conatmination-just-cutted-out.JPG | |||
File:hole-where-the contamination-was.JPG | |||
</gallery> | |||
10. Day. That Day I went to the Lab to make my own PDA-medium. To experiment more with mycelium and find out wich are the best conditions to grow it. Because i think for the paper production i need a lot more mycelium, i needs to be well moisture, for a stable growth, but nor to much medium, so that it can eat up the whole of it and i don´t have to dissolve the medium from the mycelium, because the water is not good for the mycelium either. | 10. Day. That Day I went to the Lab to make my own PDA-medium. To experiment more with mycelium and find out wich are the best conditions to grow it. Because i think for the paper production i need a lot more mycelium, i needs to be well moisture, for a stable growth, but nor to much medium, so that it can eat up the whole of it and i don´t have to dissolve the medium from the mycelium, because the water is not good for the mycelium either. | ||
| Line 86: | Line 88: | ||
Therefore i cooked some old potatoes: | Therefore i cooked some old potatoes: | ||
<gallery> | |||
File:Potatoes-cooking.JPG | |||
File:Potatoes-cooking.JPG | |||
</gallery> | |||
and boiled them for maybe 20 minutes, than took them out of the water: | and boiled them for maybe 20 minutes, than took them out of the water: | ||
| Line 107: | Line 112: | ||
I waited: | I waited: | ||
<gallery> | |||
File:Me-waiting.JPG | |||
File:Experiment-30.11.JPG | |||
</gallery> | |||
And cut out mycelium from my last experiment with a knife, disinfected with fire. With those mycelium pieces, i inoculated the PDA-medium filled petridishes. I wanted to do each in a different way, to see how i can achive the best result to produce paper from it. Therefore i varied the amount of medium and sometimes put the mycelium upside down in the medium. Additionally i wanted to know if the mycelium maybe grows better in the closet than in the oven. So i put of every kind one in the oven and one in the closet to make it comparable and marked it as you can see in the gallery below: | And cut out mycelium from my last experiment with a knife, disinfected with fire. With those mycelium pieces, i inoculated the PDA-medium filled petridishes. I wanted to do each in a different way, to see how i can achive the best result to produce paper from it. Therefore i varied the amount of medium and sometimes put the mycelium upside down in the medium. Additionally i wanted to know if the mycelium maybe grows better in the closet than in the oven. So i put of every kind one in the oven and one in the closet to make it comparable and marked it as you can see in the gallery below: | ||