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Azuzena showed us, what other people had already done. What stuck in my mind, was that you can heat mycelium and it will keep it´s form. For example in bricks or leatherlike fabric. | Azuzena showed us, what other people had already done. What stuck in my mind, was that you can heat mycelium and it will keep it´s form. For example in bricks or leatherlike fabric. | ||
Under the instructions of Azuzena, i inoculated my first medium with spores. It was 15 minutes boiled cardboard. The container we used to store it, was ironically a blue supermarket Champignon box. After the Inoculation, wich in this case only means, to put a peace of wood with spores in it, between two or three sheets of hot cardboard. With a layer of cling film we closed the container to prevent the inside from too much contamination, but than we poked holes with a fire-desinfected needle inside. Because the mycelium needs to breathe. And then leave it in the oven, that is not called oven. Jan and i had a competition who will do it better and grow it faster. Jan won, because he cared more, i was more fascinated by Azuzena and his enthusiasm about mycelium. Jan is convinced that mycelium grows better if you love it and you show your attention to it. | Under the instructions of Azuzena, i inoculated my first medium with spores. It was 15 minutes boiled cardboard. The container we used to store it, was ironically a blue supermarket Champignon box. After the Inoculation, wich in this case only means, to put a peace of wood with spores in it, between two or three sheets of hot cardboard. With a layer of cling film we closed the container to prevent the inside from too much contamination, but than we poked holes with a fire-desinfected needle inside. Because the mycelium needs to breathe. And then leave it in the oven, that is not called oven. Jan and i had a competition who will do it better and grow it faster. Jan won, because he cared more, i was more fascinated by Azuzena and his enthusiasm about mycelium. Jan is convinced that mycelium grows better if you love it and you show your attention to it. | ||
Media Mycelium grows well in: | Media Mycelium grows well in: | ||
PDY-agar (potatoestarch-dextrose-yeast) | -PDY-agar (potatoestarch-dextrose-yeast) | ||
coffee mold | |||
wood | -coffee mold | ||
cardboard (it is out of wood of course..) | |||
straw | -wood | ||
-cardboard (it is out of wood of course..) | |||
-straw | |||
Additionally it needs to be moistured regularly and grows best in a temperature between 22- 26° C. It needs also air to breathe, if you want it to crop and become fungi, it needs to be dominant over the other micro-organisms in its habit, and you have to rise the temperature i think.. | Additionally it needs to be moistured regularly and grows best in a temperature between 22- 26° C. It needs also air to breathe, if you want it to crop and become fungi, it needs to be dominant over the other micro-organisms in its habit, and you have to rise the temperature i think.. | ||
I think some humans are similar to mycelium, i could live in the same way at least for some time i think. | I think some humans are similar to mycelium, i could live in the same way at least for some time i think. | ||
[[File:Bildschirmfoto 2018-02-11 um 13.10.37.png| | [[File:Bildschirmfoto 2018-02-11 um 13.10.37.png|300px]] | ||
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While a PCR machine redoubles a part of our DNA, marked by a primer. Julian Chollet explains us the process. At first the machine heats up the small Eppis, our DNA double Helix splits up. The primer adopts to a special sequence, on each side one. | While a PCR machine redoubles a part of our DNA, marked by a primer. Julian Chollet explains us the process. At first the machine heats up the small Eppis, our DNA double Helix splits up. The primer adopts to a special sequence, on each side one. | ||
„A primer is a short strand of RNA or DNA (generally about 18-22 bases) that serves as a starting point for DNA synthesis. It is required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of DNA. The polymerase starts replication at the 3'-end of the primer, and copies the opposite strand.” (Wikipedia). | „A primer is a short strand of RNA or DNA (generally about 18-22 bases) that serves as a starting point for DNA synthesis. It is required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of DNA. The polymerase starts replication at the 3'-end of the primer, and copies the opposite strand.” (Wikipedia). | ||
Yes and then it cools the Eppendorf test tubes down and the nucleotides can be added to the strand. And this happens a lot of times (30 more or less) until there is enough DNA to be analized. | Yes and then it cools the Eppendorf test tubes down and the nucleotides can be added to the strand. And this happens a lot of times (30 more or less) until there is enough DNA to be analized. | ||
Only the sequence between the beginning and end primer is being redoubled, that makes diffrent DNA´s comparable. At least parts of it. I think Julian brought us the primer that is used to seperate the part of the Helix that stores the information about our regional origin. | Only the sequence between the beginning and end primer is being redoubled, that makes diffrent DNA´s comparable. At least parts of it. I think Julian brought us the primer that is used to seperate the part of the Helix that stores the information about our regional origin. | ||