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===Plate #6=== | ===Plate #6=== | ||
*04.11.2019 nematode medium Elliy inoculum | |||
*05.11.2019 Inoculum cube has a couple of active approx five nematodes, outside there is no e.coli growing, the coli wasn’t properly inoculated. No nematodes. | |||
<gallery> | <gallery> | ||
File:m-IMG00008.jpg|04.12.2019 | File:m-IMG00008.jpg|04.12.2019 | ||
Revision as of 21:37, 4 December 2019
Atempt to cultivate
27.11.2019 front
27.11.2019 reverse
04.12.2019 front
04.12.2019 reverse
Plate #1
- 30.10.2019 LB medium cooked by Maxi
- 01.11.2019 e.coli inoculated by Maxi
- 05.11.2019 nematode inoculum by Elliy from the original carolina plate. e.coli doesn't look grown, nematodes remain on the inoculated cube, many of them can be found.
04.12.2019
Plate #2
- 04.11.19 e.coli medium by Elliy
- 05.11.19 inoculated by Elliy with a cube of nematodes from the carolina plate
04.12.2019
Plate #3
- 04.11.19 e.coli medium; nematode inoculum Miga
- 05.11.19 One nematode on inoculated cube not moving. There is one other nematode on the e.coli medium, it is moving manically.
04.12.2019
Plate #4
- 04.11.19 e.coli medium; nematode inoculum Maggy
- 05.11.19 Nematodes don’t like the e.coli medium. They all stayed on the cube most of them are dead but some are still alive. No nematodes founds outside of the inoculated cube.
04.12.2019
Plate #5
- 04.11.2019 nematode medium. e.coli plate.
- 05.11.2019 inoculum: 1 active nematode. Outside inoculum loads of nematodes inactive. Not sterile plate.
04.12.2019
Plate #6
- 04.11.2019 nematode medium Elliy inoculum
- 05.11.2019 Inoculum cube has a couple of active approx five nematodes, outside there is no e.coli growing, the coli wasn’t properly inoculated. No nematodes.
04.12.2019
Plate #7
04.12.2019
Plate #8
04.12.2019
Plate #9
04.12.2019
Plate #10
04.12.2019
Plate #11
04.12.2019
Atempt to safe for future
C. elegans on plates #1-#11 kept in a fridge for a couple of weeks. Medium doesn't dry out and is kept fresh. Looks pretty much promising
C. elegans on plates #15-#17 kept in a fridge for a day and then in a freezer with minus 20C for 1 week. Plate with lb medium has an old smell. discarded. Other plates have perhaps living nematodes, continue being kept in a fridge.
27.11.2019. Kept in a freezer for 1 week
04.12.2019. Quite a bit of liquid in the self made lb and ngm medium. Plate with lb discarded
