GMU talk:Synthetic Biology/Bacteria Game/iGEM Wiki: Difference between revisions

Revision as of 15:28, 4 October 2010

iGem Wiki preparation page

Introduction

Bacteria Game

Small introductory text.

Description

A in depth description of the non-technical concept.

Technical Description

The concept of the game is based on the ability of some harmless wildtype bacteria to swim in soft media. Swimming enables the microbes to consume further nutrients if those in their vicinity are already consumed. All bacteria try to get away from the starting point as fast as possible to access fresh media. This mechanism can be employed for a game setup. Selection and culturing of best swimmers leads to propagation of ideal swimming characteristics. That is, why training may help gain a competitive edge. Those bacteria can easily be stored in the fridge with the supplied materials without any risk.

The showdown competition is run by synthetic bacteria. Predators and prey communicate and regulate each other's density. Via molecular signals, the predator cells kill the prey while living prey rescues predators. The diverse and colorful crowd surrounding the spectacle was genetically engineered to carry different pigments which has been appreciated at the iGEM competition 2009.

Notebook

29/10/2010

transformation of TOP10 cells with plasmids out of the registry following standard recommendations
over-night growth on selective agar plates

**table 1:** overview of used iGEM constructs
plasmid ID	Part	pigment color	backbone	registry location
pLA01	Template:Part	red	Template:Part	2010 Kit Plate 3, 6J
pLA02	Template:Part	orange	Template:Part	2010 Kit Plate 3, 6N
pLA03	Template:Part	purple	Template:Part	2010 Kit Plate 3, 12B
pLA04	Template:Part	dark green	Template:Part	2010 Kit Plate 3, 20H
pLA05	Template:Part	light green	Template:Part	2010 Kit Plate 3, 20J

**table 1:** used predator-prey system, gift from R. Smith
plasmid ID	name/function	cell strain	plasmid	marker
1a	predator	MG1655	ptetLuxRLasI-luxCcdA(SC101) placCcdBs-tetGFPuv(LVA)	Cm Kan
3a	prey	MG1655	pLasRLuxI-luxCcdBs ptet-mCherry(ColE1)	Cm Kan

@@ Line 16: / Line 16: @@
 === 29/10/2010 ===
 * [http://2010.igem.org/Transformation transformation] of TOP10 cells with plasmids out of the registry following [http://partsregistry.org/Help:Spring_2010_DNA_distribution#DNA_Kit_Plate_Instructions standard recommendations]
+* over-night growth on selective agar plates
 <br/>
@@ Line 21: / Line 22: @@
 {| class="wikitable sortable" , border="1"
 |- bgcolor=grey
-! height=20px, align='center', width=80px | plasmid ID || width=150px | Part || width=150px | pigment color || width=140 px | backbone || width=250 | registry location
+! height=20px, align='center', width=80px | plasmid ID || width=150px | Part || width=150px | pigment color || width=150 px | backbone || width=250 | registry location
 |-style="height:20px"
-|+ align="bottom" |''<b>table 1:</b>''
+|+ align="bottom" |''<b>table 1:</b>'' overview of used iGEM constructs
 |-align="center"
 | style="font-weight:bold;" | pLA01 || {{Part|BBa_K274110}} || red  || {{Part|pSB1A2}} || 2010 Kit Plate 3, 6J
+|-align="center"
+| style="font-weight:bold;" | pLA02 || {{Part|BBa_K274210}} || orange  || {{Part|pSB1A2}} || 2010 Kit Plate 3, 6N
+|-align="center"
+| style="font-weight:bold;" | pLA03 || {{Part|BBa_K274002}} || purple  || {{Part|pSB1T3}} || 2010 Kit Plate 3, 12B
+|-align="center"
+| style="font-weight:bold;" | pLA04 || {{Part|BBa_K274003}} || dark green  || {{Part|pSB1K3}} || 2010 Kit Plate 3, 20H
+|-align="center"
+| style="font-weight:bold;" | pLA05 || {{Part|BBa_K274004}} || light green  || {{Part|pSB1K3}} || 2010 Kit Plate 3, 20J
+|}
+<br/>
+{| class="wikitable sortable" , border="1"
+|- bgcolor=grey
+! height=20px, align='center', width=80px | plasmid ID || width=150px | name/function || width=150px | cell strain || width=350 px | plasmid || width=100 | marker
+|-style="height:20px"
+|+ align="bottom" |''<b>table 1:</b>'' used predator-prey system, gift from R. Smith
+|-align="center"
+| style="font-weight:bold;" | 1a || predator || MG1655  || ptetLuxRLasI-luxCcdA(SC101) <br/> placCcdBs-tetGFPuv(LVA) || Cm <br/> Kan
+|-align="center"
+| style="font-weight:bold;" | 3a || prey || MG1655  || pLasRLuxI-luxCcdBs <br/> ptet-mCherry(ColE1) || Cm <br/> Kan
 |}