GMU talk:Synthetic Biology/Bacteria Game/iGEM Wiki: Difference between revisions
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== Notebook == | == Notebook == | ||
=== 29/10/2010 === | === 29/10/2010 === | ||
* [http://2010.igem.org/Transformation transformation] of TOP10 cells with plasmids out of the registry following [http://partsregistry.org/Help:Spring_2010_DNA_distribution#DNA_Kit_Plate_Instructions standard recommendations] | * [http://2010.igem.org/Transformation transformation] of TOP10 cells with plasmids (see table 1) out of the registry following [http://partsregistry.org/Help:Spring_2010_DNA_distribution#DNA_Kit_Plate_Instructions standard recommendations] | ||
* over-night growth on selective agar plates | * over-night growth on selective agar plates | ||
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{| class="wikitable sortable" , border="1" | {| class="wikitable sortable" , border="1" | ||
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! height=20px, align='center', width=80px | plasmid|| width=150px | Part || width=150px | pigment color || width=150 px | backbone || width= | ! height=20px, align='center', width=80px | plasmid|| width=150px | Part || width=150px | pigment color || width=150 px | backbone || width=150 | registry location | ||
|-style="height:20px" | |-style="height:20px" | ||
|+ align="bottom" |''<b>table 1:</b>'' overview of used iGEM constructs | |+ align="bottom" |''<b>table 1:</b>'' overview of used iGEM constructs | ||
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* glycerol stocks (prepared directly after strains were received) from predator and prey cells (see table 2) were plated on selective agar plates | |||
<br/> | <br/> | ||
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! height=20px, align='center', width=80px | sample || width=150px | name/function || width=150px | cell strain || width=350 px | plasmid || width=100 | marker | ! height=20px, align='center', width=80px | sample || width=150px | name/function || width=150px | cell strain || width=350 px | plasmid || width=100 | marker | ||
|-style="height:20px" | |-style="height:20px" | ||
|+ align="bottom" |''table | |+ align="bottom" |''table 2:'' used predator-prey system, gift from R. Smith | ||
|-align="center" | |-align="center" | ||
| style="font-weight:bold;" | 1a || predator || MG1655 || ptetLuxRLasI-luxCcdA(SC101), placCcdBs-tetGFPuv(LVA) || Cm, Kan | | style="font-weight:bold;" | 1a || predator || MG1655 || ptetLuxRLasI-luxCcdA(SC101), placCcdBs-tetGFPuv(LVA) || Cm, Kan | ||
Revision as of 15:35, 4 October 2010
iGem Wiki preparation page
Introduction
Small introductory text.
Description
A in depth description of the non-technical concept.
Technical Description
The concept of the game is based on the ability of some harmless wildtype bacteria to swim in soft media. Swimming enables the microbes to consume further nutrients if those in their vicinity are already consumed. All bacteria try to get away from the starting point as fast as possible to access fresh media. This mechanism can be employed for a game setup. Selection and culturing of best swimmers leads to propagation of ideal swimming characteristics. That is, why training may help gain a competitive edge. Those bacteria can easily be stored in the fridge with the supplied materials without any risk.
The showdown competition is run by synthetic bacteria. Predators and prey communicate and regulate each other's density. Via molecular signals, the predator cells kill the prey while living prey rescues predators. The diverse and colorful crowd surrounding the spectacle was genetically engineered to carry different pigments which has been appreciated at the iGEM competition 2009.
Notebook
29/10/2010
- transformation of TOP10 cells with plasmids (see table 1) out of the registry following standard recommendations
- over-night growth on selective agar plates
| plasmid | Part | pigment color | backbone | registry location |
|---|---|---|---|---|
| pLA01 | Template:Part | red | Template:Part | 2010 Kit Plate 3, 6J |
| pLA02 | Template:Part | orange | Template:Part | 2010 Kit Plate 3, 6N |
| pLA03 | Template:Part | purple | Template:Part | 2010 Kit Plate 3, 12B |
| pLA04 | Template:Part | dark green | Template:Part | 2010 Kit Plate 3, 20H |
| pLA05 | Template:Part | light green | Template:Part | 2010 Kit Plate 3, 20J |
- glycerol stocks (prepared directly after strains were received) from predator and prey cells (see table 2) were plated on selective agar plates
| sample | name/function | cell strain | plasmid | marker |
|---|---|---|---|---|
| 1a | predator | MG1655 | ptetLuxRLasI-luxCcdA(SC101), placCcdBs-tetGFPuv(LVA) | Cm, Kan |
| 3a | prey | MG1655 | pLasRLuxI-luxCcdBs, ptet-mCherry(ColE1) | Cm, Kan |