GMU:DIY Biolab “Driver’s License”/Mana Mazidy

Glowing Banknote tell the true story.

A banknote is a document that serves as a means of payment in a particular country. The banknote is a small poster that tells the story of a country. In this project we try to show that the true story is hidden behind the beautiful photo. Behind the laughing faces of politicians, men scream for help, crying the mothers and trembling children. Here you will not see famous politicians, people laughing and beautiful nature, but you can see the sad story that is happening right now!

The banknotes are public media. it means everyone knows them! You do not need to go to the museum to see them. that's why we think of it as a very powerful medium and want to use it to tell the true story of the countries!

[1]

But how?

The publishers of banknotes try to make their banknotes difficult to copy. They use different techniques to control the correct banknotes One of the techniques is: Fluorescent colors It is used printing ink containing fluorescent pigments. In short-wave UV light, depending on the choice of pigment, colors are emitted in the visible light spectrum. In this project instead of pigments we use bacteria to draw our pictures. So we have a connection between human, media and bacteria!

[2]

Which bacteria?

bioluminescence

Bioluminescence is the production and emission of light by a living organism. It is a form of chemiluminescence. Bioluminescence is a form of chemiluminescence where light energy is released by a chemical reaction. This reaction involves a light-emitting pigment, the luciferin, and a luciferase, the enzyme component.

So we have a connection between humans, media and bacteria. You can see the normal photos on banknote, but when the light is off, the bacteria will glow and you'll see glowing photos telling the right story.

[3]

bioluminescent chemicals

.......................................................................................................

Preparing to grow Bioluminesence algae

1-Some oft hem are easier to see and produce brighter flashes than other

2-I orderd it by Carolina.com

3-Algae need plenty of light and nutrients to multiply in the osean.

4-We need Algae rowth Solution . Should be a natural solution made with real seawater

5-we need a clear container (glass or Plastik)

6-A 40Watt lamp

7-a place temrature = 22-25C

8-Cleaning no soap sterlize it

• they getting schocked by temprature change*

• Dont allow the Solution to sit in the sun*

9-12Uhr light

• Light should be placed about tree feet from the comtainer*

10-tern on the light a the same time in the morning and turn off at the same time at night

11_simulate the algae = it glow

• Dinoflagellates flash when water on stimulates them by pressing against their cell walles*

• the luminesences wont be as bright at first . since it will take a few weeks fort he algae population to restore itself*

Ability

they only glow when they are alive . so you can know are they dead or alive ! in this project we can use this Ability to draw . The positive area should be filled with live bacteria and negative area with dead bacteria.

Which countries Which banknotes

we want to tell the story of the countries where currently in 2017-2018 goes wrong. War. Diseases . Poverty ... In this time there are many countries where there are war or countries in which the children starve to death. Some countries are economically blocked and do not get any medicines, so many die after illnesses.

'Everything for money and power.'

.........................................................................

Make Bacteria glowing :

bacteria need food to grow It should to be full of vitamins, amino acids, carbon, minerals and of course salt, without salt they won't live !

6.Desember

I tryed to Make Some Bacteria Food :

• TSA agar gel*

what I need : NaCI 2g . Yeast Extract 1g . Peptone 2g . Agar 3g . Fill with H2O up till 200 ml

+ Photobacterium

What ist Photobacterium

Tehy are a genus of Gram-negative bacteria and belong to the family of Vibrionaceae. Some members of this genus have the ability to produce light and are therefore bioluminescent

7.Desember

it is not glowing :(

14.Desember

they are growing but not glowing

20.Desember

I use this bacteria to draw

I want to see how many details are visible when they are growing

......................................................................................

......................................................................................

......................................................................................

Same projects:

[4]https://bscd.uchicago.edu/news/student-project-glowing-bacteria-art-gallery

Student project of chicago university fluoresced bacteria - meta-bacterial

......................................................................................

[5]http://flavorwire.com/398549/glow-in-the-dark-art-created-in-the-lab-with-bioluminescent-bacteria/view-all

With Bioluminescent Bacteria - gallery at Montana State University

........................................................................................

[6]http://www.miyaando.com/installations-1/2016/12/8/amwdv9kbdzbcliqsfs211iazo1pdiy

from artist Miya Ando and photographer L. Young. With a different kind of bioluminescence

........................................................................................

[7]http://2012.igem.org/Team:Caltech/Human_Practice

California Institute of the Arts to create animations from fluorescent bacteria

........................................................................................

[8]https://www.huffingtonpost.com/entry/agar-art-contest_us_56253e13e4b02f6a900d3d57

composed of multiple petri dishes of bacteria modified with fluorescent protein under UV light

.........................................................................................

Modification of green fluorescent

I noticed that most artists used either bioluminecenses or fluresences bactera.

But what's new: modified with fluorescent protein! I tried to know, what is it? and how can I do that?

GFP means green fluorescent protein. most are from jellyfish! the artists have used it for art projects and scientists have used it to highlighting specific structures.