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==HUMAN DNA ANALYSIS== | |||
[[File:Blood collected2.jpg|400px]] | [[File:Blood collected2.jpg|400px]] | ||
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==PRACTICING PCR ANALYSIS== | |||
Step 1. Extracting DNA from blood and from pork liver meat | ===Step 1. Extracting DNA from blood and from pork liver meat=== | ||
[[File:STEP 1.1.jpg|400px]] | [[File:STEP 1.1.jpg|400px]] | ||
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Step 2. STR-analysis with pork meat primer | ===Step 2. STR-analysis with pork meat primer=== | ||
[[File:STEP 2.1.jpg|400px]] | [[File:STEP 2.1.jpg|400px]] | ||
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Step 3. Electrophoresis | ===Step 3. Electrophoresis=== | ||
= | ====#First attempt==== | ||
[[File:STEP 3.1.JPG|400px]] | [[File:STEP 3.1.JPG|400px]] | ||
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====#Second attempt==== | |||
= | |||
[[File:STEP 3.2.1.jpg|400px]] | [[File:STEP 3.2.1.jpg|400px]] | ||
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====#Third attempt==== | |||
= | |||
[[File:STEP 3.3.1.jpg|400px]] | [[File:STEP 3.3.1.jpg|400px]] | ||
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====#Fourth attempt==== | |||
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[[File:STEP 3.4.1.jpg|400px]] | [[File:STEP 3.4.1.jpg|400px]] | ||
Revision as of 20:37, 23 January 2018
HUMAN DNA ANALYSIS
PRACTICING PCR ANALYSIS
Step 1. Extracting DNA from blood and from pork liver meat
Step 2. STR-analysis with pork meat primer
Step 3. Electrophoresis
#First attempt
=> I got no results : either the ladder nor the DNA samples were "growing" as they should (multiple lines). So I decided to test the ladder only to see if there is a problem from the samples themselves or with the preparation (agarose + buffer TAE 50x+ distilled water) => #Second attempt
#Second attempt
=> Same problem : the ladder was growing as one bloc only (see the little drawing). Even during a 2.2 attempt with new SERVA Stain G (which I thought it could be the problem, since the previous tube was quiet old). Then, I noticed that the positive side was turning in blue color and some part of the agarose gel was melting down. So I decided to test if this bleu color is coming from oxidation of the electrodes themselves, or if the ladder was "swimming" on the extra amount of solution (buffer TAE 50x + distilled water) above the agarose to reach the positive side => #Third attempt
#Third attempt
=> Still turning bleu. Even with smaller electrodes that are completely covered by the solution (buffer TAE 50x + distilled water) So I decided to test if this oxidation is coming from the buffer or if it is simply a natural process => #Fourth attempt
#Fourth attempt
=> First picture : distilled water only. Second picture : distilled water + buffer TAE 50x. No changes at all. So it might come from the SERVA Stain G (which is yellow-ish) or it is actually not a problem and does not affect on the electrophoresis itself.