GMU:Introduction to Microscopy/Denise Nicoau: Difference between revisions

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''23/11/2020''
''23/11/2020''


==='''Research: Nematodes - tiny parasites'''===
==='''Research: Nematodes'''===


''In short, if all the matter in the universe except the nematodes were swept away, our world would still be dimly recognizable, and if, as disembodied spirits, we could then investigate it, we should find its mountains, hills, vales, rivers, lakes, and oceans represented by a film of nematodes.'' - by Nathan Cobb - nematologist.
''In short, if all the matter in the universe except the nematodes were swept away, our world would still be dimly recognizable, and if, as disembodied spirits, we could then investigate it, we should find its mountains, hills, vales, rivers, lakes, and oceans represented by a film of nematodes.'' - by Nathan Cobb - nematologist.
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'''4 out of every 5 animals that live our planet are nematodes.'''  
'''4 out of every 5 animals that live our planet are nematodes.'''  


Nematodes are multicellular insects, tiny roundworms who inhabit a broad range of environments; they are parasitic and free-living organisms that feed on living material (e.g. plants, insects or other nematodes). Nematodes have successfully adapted to nearly every ecosystem (soil, freshwaters, tropics, polar regions, ...), in fact they can regulate bacterial population and community composition (they may eat up to 5,000 bacteria per minute!). They can be also hermaphrodites, capable of self fertilization.  
Nematodes are multicellular roundworms who inhabit a broad range of environments; there are many types of nematodes, some are parasitic and free-living organisms that feed on living material (e.g. plants, insects or other nematodes). Nematodes have successfully adapted to nearly every ecosystem (soil, freshwaters, tropics, polar regions, ...), in fact they can regulate bacterial population and community composition (they may eat up to 5,000 bacteria per minute!). They can be also hermaphrodites, capable of self fertilization.  


Important role played in the decomposition process. Sensitive to pollution.
Important role played in the decomposition process. Sensitive to pollution.
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From a pond water sample, it has been found a nematode whose body had been trapped by a fungus. The fungus first punctured and expanded, paralyzing the nematode. Then he fed himself quickly, digesting the nematode's body from within.  
From a pond water sample, it has been found a nematode whose body had been trapped by a fungus. The fungus first punctured and expanded, paralyzing the nematode. Then he fed himself quickly, digesting the nematode's body from within.  


''25/11/2020''
''25/11/2020''
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As I would like to explore a bit more wet mounts and living microorganisms, I will start with some pond water that I will examine with the binocular microscope.
As I would like to explore a bit more wet mounts and living microorganisms, I will start with some pond water that I will examine with the binocular microscope.


I decided to collect some pond water samples in weimar: I picked some drops in different parts of the pond and then stored them in 4 pipettes to compare them. I prepared the specimens and then waited from 30min to 1 hour to examine all of them, in order to let them familiarize with the environment again.
I decided to collect some pond water samples in weimar: I picked some drops in different parts of the pond and then stored them in 4 tubes to compare them. I prepared the specimens and then waited from 30min to 1 hour to examine all of them, in order to let them familiarize with the environment again.


''Overall:'' a very few moving microorganisms and plenty of static microorganisms.  
''Overall:'' a very few moving microorganisms and plenty of static microorganisms/segments of plants.  
Were they dead or just asleep? 


Here's what I found:
Here's what I found:


'''Pipette 1:'''
'''Tube 1:'''


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'''Pipette 2:'''
'''Tube 2:'''


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'''Pipette 3:'''
'''Tube 3:'''


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'''Pipette 4:'''
'''Tube 4:'''


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