Fomes fomentarius (Tinder Mushroom): Difference between revisions

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==== '''Day 1 Prepare agar medium flat jars''' ====

Objective: Prepare a versatile nutritional medium for mushroom cultivation.

Materials: Flat jar, wadding, agar-agar, malt extract powder, marker, distilled water, pressure cooker, precision scale (0,1 g), aluminum foil.

Part 1: Calculate Ingredient Ratios

{| class="wikitable"

|+

!Component

!Quantity

!

!Custom Quantity

|-

|Malt Extract (Nutrient source)

|2g

|x

|

|-

|Agar-agar (Footing for the growth - 3D support)

|2g

|x

|

|-

|Distilled water

|100ml

|x

|

|}

Part 2: Weigh Ingredients And Mix Into Flask

* Use a scale to measure the required ingredients accurately.

* Construct a container using clean aluminum foil or paper.

* Combine the ingredients in a flask or jar, ensuring thorough mixing.

* Create a hole in the lid and insert cotton wadding to enable pressure cooking and facilitate air exchange for the mushrooms.

* Pour enough of the mixture into each container to cover the bottom adequately. (A thicker agar layer consumes more materials but prevents drying during long-term fridge storage.)

* Seal the jars and cover the lids with aluminum foil to prevent microbes from reaching the air filter later on.

Part 3: Sterilize In Pressure Cooker

* Add a few centimeters of water into the pressure cooker, ensuring the vessels are submerged but not floating. Close the cooker and reach the designated temperature and pressure point (121°C, 15 PSI). (Avoid using airtight containers in the pressure cooker to prevent bursting; always refer to the pressure cooker manual for proper usage.)

* Reduce the heat to maintain a gentle boil and keep it at the minimum temperature to ensure the water continues to boil.

* Wait for 40 minutes from the point when pressure and heat are reached.

* Turn off the heating source and allow the cooker to cool down naturally. HINT: Avoid releasing pressure suddenly as it may cause the medium to overboil.

* Once the cooker is slightly warm, release the remaining pressure (there should be none), open the lid, and carefully remove the medium.

* Let the jars cool down naturally before storing them in the fridge.

Part 4: Add Mycelium

For reference to the original experiment, please see [https://www.top-ev.de/wp-content/uploads/2021/12/Prepare-agar-medium-flat-jars-v.2.0.pdf Prepare agar medium flat jars]

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==== '''Day 3 Preparation of a Small Bag with Substrate''' ====

Objective: Prepare a sterile small bag to expand mycelium for fructification, fabrication, or as an inoculant for larger vessels.

Materials for 1 bag: See below, bucket that can hold 5 L (for mixing), jar with clean grain spawn, substrate, '''alcohol(?)''', autoclavable plastic bags, gloves, distilled water (approx. 2,5x the dry weight of the substrate).

For reference to the original experiment, please see [https://www.top-ev.de/wp-content/uploads/2021/12/Prepare-small-mycelium-bags-v.2.0-1.pdf Preparation of a Small Bag with Substrate]

==== '''Day 4 Fabrication of a solid object made of mycelium composite materials''' ====

For reference to the original experiment, please see [https://www.top-ev.de/wp-content/uploads/2021/09/Fabrication-of-a-solid-object-made-of-mycelium.pdf Fabrication of a solid object made of mycelium composite materials]

@@ Line 15: / Line 15: @@
 ==== '''Day 1 Prepare agar medium flat jars''' ====
+Objective: Prepare a versatile nutritional medium for mushroom cultivation.
+Materials: Flat jar, wadding, agar-agar, malt extract powder, marker, distilled water, pressure cooker, precision scale (0,1 g), aluminum foil.
+<u>Part 1: Calculate Ingredient Ratios</u>
+{| class="wikitable"
+|+
+!Component
+!Quantity
+!
+!Custom Quantity
+|-
+|Malt Extract (Nutrient source)
+|2g
+|x
+|
+|-
+|Agar-agar (Footing for the growth - 3D support)
+|2g
+|x
+|
+|-
+|Distilled water
+|100ml
+|x
+|
+|}
+<u>Part 2: Weigh Ingredients And Mix Into Flask</u>
+* Use a scale to measure the required ingredients accurately.
+* Construct a container using clean aluminum foil or paper.
+* Combine the ingredients in a flask or jar, ensuring thorough mixing.
+* Create a hole in the lid and insert cotton wadding to enable pressure cooking and facilitate air exchange for the mushrooms.
+* Pour enough of the mixture into each container to cover the bottom adequately. (A thicker agar layer consumes more materials but prevents drying during long-term fridge storage.)
+* Seal the jars and cover the lids with aluminum foil to prevent microbes from reaching the air filter later on.
+<u>Part 3: Sterilize In Pressure Cooker</u>
+* Add a few centimeters of water into the pressure cooker, ensuring the vessels are submerged but not floating. Close the cooker and reach the designated temperature and pressure point (121°C, 15 PSI). (Avoid using airtight containers in the pressure cooker to prevent bursting; always refer to the pressure cooker manual for proper usage.)
+* Reduce the heat to maintain a gentle boil and keep it at the minimum temperature to ensure the water continues to boil.
+* Wait for 40 minutes from the point when pressure and heat are reached.
+* Turn off the heating source and allow the cooker to cool down naturally. HINT: Avoid releasing pressure suddenly as it may cause the medium to overboil.
+* Once the cooker is slightly warm, release the remaining pressure (there should be none), open the lid, and carefully remove the medium.
+* Let the jars cool down naturally before storing them in the fridge.
+<u>Part 4: Add  Mycelium</u>
 For reference to the original experiment, please see [https://www.top-ev.de/wp-content/uploads/2021/12/Prepare-agar-medium-flat-jars-v.2.0.pdf Prepare agar medium flat jars]
@@ Line 84: / Line 131: @@
 ==== '''Day 3 Preparation of a Small Bag with Substrate''' ====
+Objective: Prepare a sterile small bag to expand mycelium for fructification, fabrication, or as an inoculant for larger vessels.
+Materials for 1 bag: See below, bucket that can hold 5 L (for mixing), jar with clean grain spawn, substrate, '''alcohol(?)''', autoclavable plastic bags, gloves, distilled water (approx. 2,5x the dry weight of the substrate).
 For reference to the original experiment, please see [https://www.top-ev.de/wp-content/uploads/2021/12/Prepare-small-mycelium-bags-v.2.0-1.pdf Preparation of a Small Bag with Substrate]
 ==== '''Day 4 Fabrication of a solid object made of mycelium composite materials''' ====
 For reference to the original experiment, please see [https://www.top-ev.de/wp-content/uploads/2021/09/Fabrication-of-a-solid-object-made-of-mycelium.pdf Fabrication of a solid object made of mycelium composite materials]