Fomes fomentarius (Tinder Mushroom): Difference between revisions

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Step 1: Weigh the Substrate

* Combine the solid components in a bowl and weigh them.

* Add distilled water to the mixture.

* Mix thoroughly, ensuring to break down any chunks and evenly distribute the moisture throughout the substrate.

* Transfer the substrate into a filter bag or an autoclavable container equipped with an air filter.

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Step 2: Sterilise

* Sterilize the bag by pressure cooking it for 50 minutes.

* After sterilization, allow the bag to cool down. Note that the substrate can retain heat for several hours, so always squeeze it gently to assess the temperature at the center.

Step 3: Mix In Mycelium

* If using grain spawn, break it down by shaking the jar or by using a sterile or flamed spoon to mix the grains.

* If the liquid culture is not already fragmented, mix the contents well with a sterile or flamed spoon.

* Pour or spread the inoculant into the bag and seal it.

* Roll the bag to thoroughly mix the contents.

Step 4: Incubate

* Incubate in darkness for 1 to 6 weeks.

* Check the progress of the mycelium every two to three days, and monitor for any signs of contamination.

For reference to the original experiment, please see [https://www.top-ev.de/wp-content/uploads/2021/12/Prepare-small-mycelium-bags-v.2.0-1.pdf Preparation of a Small Bag with Substrate]

@@ Line 155: / Line 155: @@
 <u>Step 1: Weigh the Substrate</u>
+* Combine the solid components in a bowl and weigh them.
+* Add distilled water to the mixture.
+* Mix thoroughly, ensuring to break down any chunks and evenly distribute the moisture throughout the substrate.
+* Transfer the substrate into a filter bag or an autoclavable container equipped with an air filter.
 {| class="wikitable"
 |+
@@ Line 212: / Line 218: @@
 |}
 <u>Step 2: Sterilise</u>
+* Sterilize the bag by pressure cooking it for 50 minutes.
+* After sterilization, allow the bag to cool down. Note that the substrate can retain heat for several hours, so always squeeze it gently to assess the temperature at the center.
 <u>Step 3: Mix In Mycelium</u>
+* If using grain spawn, break it down by shaking the jar or by using a sterile or flamed spoon to mix the grains.
+* If the liquid culture is not already fragmented, mix the contents well with a sterile or flamed spoon.
+* Pour or spread the inoculant into the bag and seal it.
+* Roll the bag to thoroughly mix the contents.
 <u>Step 4: Incubate</u>
+* Incubate in darkness for 1 to 6 weeks.
+* Check the progress of the mycelium every two to three days, and monitor for any signs of contamination.
 For reference to the original experiment, please see [https://www.top-ev.de/wp-content/uploads/2021/12/Prepare-small-mycelium-bags-v.2.0-1.pdf Preparation of a Small Bag with Substrate]