GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata: Difference between revisions
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= A visual representation of bacterial communication systems = | == A visual representation of bacterial communication systems == | ||
==Streptomyces isolation from pure soil== | ===STREPTOMYCES=== | ||
====1st step: Streptomyces isolation from pure soil==== | |||
I. Air dry the soil samples for '''3-4 h at 45°''' to select for Actinomyces spores, considering that many other bacteria die during this pre-treatment | I. Air dry the soil samples for '''3-4 h at 45°''' to select for Actinomyces spores, considering that many other bacteria die during this pre-treatment | ||
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Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria | Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria | ||
====2nd step: Adding beef extract==== | |||
The beef extract is added in order to identify the possible communication pathways of Streptomyces when it comes to nutrition. | |||
===PAENIBACILLUS VORTEX=== | |||
''Currently waiting for the bacterial strain to get delivered from Jena'' | |||
# Considering the pattern-forming abilities of ''Paenibacillus Vortex'', I am interested in depicting the possible networks this bacterium can build through quorum sensing, when it comes to the presence of nutrition. Known as one of the most intelligent bacterium species due to its advanced quorum sensing, PV might as well be the proper organism to give clear and differential communication structures. | |||
====1st step: Isolation and incubation==== | |||
====2nd step: Adding Pepton==== | |||