GMU:DIY Biolab “Driver’s License”/Suna Yoo: Difference between revisions

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Azuzena showed us, what other people had already done. What stuck in my mind, was that you can heat mycelium and it will keep it´s form. For example in bricks or leather.
Azuzena showed us, what other people had already done. What stuck in my mind, was that you can heat mycelium and it will keep it´s form. For example in bricks or leather.
Under the instructions of Azuzena i inoculated my first medium with spores. It was 15 minutes boiled cardboard. The container we used to store it, was ironically a blue supermarket Champignon box. After the Inoculation, wich in this case only means, to put a peace of wood with spores in it, between two or three sheets of hot cardboard. And then leave it in the oven, that is not called oven.
Under the instructions of Azuzena i inoculated my first medium with spores. It was 15 minutes boiled cardboard. The container we used to store it, was ironically a blue supermarket Champignon box. After the Inoculation, wich in this case only means, to put a peace of wood with spores in it, between two or three sheets of hot cardboard. And then leave it in the oven, that is not called oven.
here will be a photo of the fungi I plucked


4. 31.10. Halloween. Motivated by the experiences in the last lesson, i started doing something at home. This time i wanted to experiment with growing mycelium in coffee mold. I already had collected some for a time, because on my travels during the summer i had learned to use it instead of dish-soap. In addition i had plucked some fungi caps out of my garden, put them on a piece of paper and waited for them to drop their spores, facing their dead by drying out in my room.
4. 31.10. Halloween. Motivated by the experiences in the last lesson, i started doing something at home. This time i wanted to experiment with growing mycelium in coffee mold. I already had collected some for a time, because on my travels during the summer i had learned to use it instead of dish-soap. In addition i had plucked some fungi caps out of my garden, put them on a piece of paper and waited for them to drop their spores, facing their dead by drying out in my room.
So i took put on my new rollerskates (2) and desinfected the whole kitchen with Korn (3), a typical thuringian alcohol. Then I boiled the coffee mold (4) for 15 minutes, maybe longer and put it in a bowl to let it cool down (5). Then i desinfected an old paprika sauce glass with Korn and put the boiled mold inside (6) rotatory with pieces of the dropped spores (7&8-10) and tapped it with kitchen paper. In the end i heated my oven to 50 degrees, let it cool down and then stored the glass in it with circulating air (11). Later that day i went to the lab to put in in the real machine.
So i took put on my new rollerskates (3) and desinfected the whole kitchen with Korn (4), a typical thuringian alcohol. Then I boiled the coffee mold (5) for 15 minutes, maybe longer and put it in a bowl to let it cool down (6). Then i desinfected an old paprika sauce glass with Korn and put the boiled mold inside (7) rotatory with pieces of the dropped spores (8&9-11) and tapped it with kitchen paper. In the end i heated my oven to 50 degrees, let it cool down and then stored the glass in it with circulating air (12). Later that day i went to the lab to put in in the real machine.
 
Here will be the pictures 3-12


5. Day. We started a Analization of our DNA. Means: gargle a sodium solution and then pipet a lot. I don´t know what and maybe even Miga does not really know. We follow the instruction. I think we seperated our DNA..
5. Day. We started a Analization of our DNA. Means: gargle a sodium solution and then pipet a lot. I don´t know what and maybe even Miga does not really know. We follow the instruction. I think we seperated our DNA..
This is a photo of my last experiment, 1 week later:
This is a photo of my last experiment, 1 week later:
Photo


I think it is contaminated, definetly too wet, says Miga.
I think it is contaminated, definetly too wet, says Miga.
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Well i had a lot of worries. And yes not everything is really working how i thought, but i am still in the process.
Well i had a lot of worries. And yes not everything is really working how i thought, but i am still in the process.


 
2 Photos of that Day
 


9. 30.11.17  I met with Jan in the Lab, because he had a lot of PDA (Potatostarch-Dextrose-Agar) medium, he offered me to use for the first try.
9. 30.11.17  I met with Jan in the Lab, because he had a lot of PDA (Potatostarch-Dextrose-Agar) medium, he offered me to use for the first try.
I don´t have Photos of that Day, but here is, what came out a week later:
I don´t have Photos of that Day, but here is, what came out a week later:
Photo


It was already contaminated (the little greenish dots) and began to roll up (in the upper right corner).
It was already contaminated (the little greenish dots) and began to roll up (in the upper right corner).