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here will be a photo of the fungi I plucked | here will be a photo of the fungi I plucked | ||
4. 31.10. Halloween. Motivated by the experiences in the last lesson, i started doing something at home. This time i wanted to experiment with growing mycelium in coffee mold. I already had collected some for a time, because on my travels during the summer i had learned to use it instead of dish-soap. In addition i had plucked some fungi caps out of my garden, put them on a piece of paper and waited for them to drop their spores, facing their dead by drying out in my room. | 4. Day 31.10. Halloween. Motivated by the experiences in the last lesson, i started doing something at home. This time i wanted to experiment with growing mycelium in coffee mold. I already had collected some for a time, because on my travels during the summer i had learned to use it instead of dish-soap. In addition i had plucked some fungi caps out of my garden, put them on a piece of paper and waited for them to drop their spores, facing their dead by drying out in my room. | ||
So i took put on my new rollerskates (3) and desinfected the whole kitchen with Korn (4), a typical thuringian alcohol. Then I boiled the coffee mold (5) for 15 minutes, maybe longer and put it in a bowl to let it cool down (6). Then i desinfected an old paprika sauce glass with Korn and put the boiled mold inside (7) rotatory with pieces of the dropped spores (8&9-11) and tapped it with kitchen paper. In the end i heated my oven to 50 degrees, let it cool down and then stored the glass in it with circulating air (12). Later that day i went to the lab to put in in the real machine. | So i took put on my new rollerskates (3) and desinfected the whole kitchen with Korn (4), a typical thuringian alcohol. Then I boiled the coffee mold (5) for 15 minutes, maybe longer and put it in a bowl to let it cool down (6). Then i desinfected an old paprika sauce glass with Korn and put the boiled mold inside (7) rotatory with pieces of the dropped spores (8&9-11) and tapped it with kitchen paper. In the end i heated my oven to 50 degrees, let it cool down and then stored the glass in it with circulating air (12). Later that day i went to the lab to put in in the real machine. | ||
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I think it is contaminated, definetly too wet, says Miga. | I think it is contaminated, definetly too wet, says Miga. | ||
6. While a PCR machine redoubles a part of our DNA, marked by a primer. Julian explains us the process (13). At first the machine heats up the small Eppis, our DNA double Helix splits up. The primer adopts to a special sequence, on each side one. „A primer is a short strand of RNA or DNA (generally about 18-22 bases) that serves as a starting point for DNA synthesis. It is required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of DNA. The polymerase starts replication at the 3'-end of the primer, and copies the opposite strand.” (Wikipedia). | 6. Day. While a PCR machine redoubles a part of our DNA, marked by a primer. Julian explains us the process (13). At first the machine heats up the small Eppis, our DNA double Helix splits up. The primer adopts to a special sequence, on each side one. „A primer is a short strand of RNA or DNA (generally about 18-22 bases) that serves as a starting point for DNA synthesis. It is required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of DNA. The polymerase starts replication at the 3'-end of the primer, and copies the opposite strand.” (Wikipedia). | ||
Yes and then it cools the Eppi down and the nucleotides can be added to the strand. And this happens a lot of times (30 more or less) until there is enough to be analized. | Yes and then it cools the Eppi down and the nucleotides can be added to the strand. And this happens a lot of times (30 more or less) until there is enough to be analized. | ||
Only the sequence between the beginning and end primer is being redoubled, that makes diffrent DNA´s comparable. At least parts of it. I think Julian brought us the primer that is used to seperate the part of the Helix that stores the information about our regional origin. | Only the sequence between the beginning and end primer is being redoubled, that makes diffrent DNA´s comparable. At least parts of it. I think Julian brought us the primer that is used to seperate the part of the Helix that stores the information about our regional origin. | ||
7. Today we wanted to use gel electrophoresis to compare and analize our DNA, but it didn´t work. We don´t know why. | 7. Day. Today we wanted to use gel electrophoresis to compare and analize our DNA, but it didn´t work. We don´t know why. | ||
8. We have to present our ideas. | 8. Day. We have to present our ideas. | ||
Well i had a lot of worries. And yes not everything is really working how i thought, but i am still in the process. | Well i had a lot of worries. And yes not everything is really working how i thought, but i am still in the process. | ||
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2 Photos of that Day | 2 Photos of that Day | ||
9. 30.11.17 I met with Jan in the Lab, because he had a lot of PDA (Potatostarch-Dextrose-Agar) medium, he offered me to use for the first try. | 9. Day. 30.11.17 I met with Jan in the Lab, because he had a lot of PDA (Potatostarch-Dextrose-Agar) medium, he offered me to use for the first try. | ||
I don´t have Photos of that Day, but here is, what came out a week later: | I don´t have Photos of that Day, but here is, what came out a week later: | ||
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[[File:hole-where-the contamination-was.JPG|400px]] | [[File:hole-where-the contamination-was.JPG|400px]] | ||
10. That Day I went to the Lab to make my own PDA-medium. Therefore i cooked some old potatoes: | 10. Day. That Day I went to the Lab to make my own PDA-medium. Therefore i cooked some old potatoes: | ||
[[File:Potatoes-cooking.JPG|400px]][[File:Potatoes-cooking.JPG|400px]] | [[File:Potatoes-cooking.JPG|400px]][[File:Potatoes-cooking.JPG|400px]] | ||