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=== | ===ISOLATION OF STREPTOMYCES=== | ||
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment==== | |||
====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min==== | |||
====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it==== | ====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it==== |
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