Paola Stephania

BACKGROUND.

• Architect and Designer with an emphasis in product design of Los Andes University (Bogotá/Colombia)
• Currently making Master in Media Architecture in Bauhaus University (Weimar/Germany)

PROJECT IDEA.

• Project idea 1: Create a series of photographies of a variety of specimens out and under the microscope. The specimens can come from laboratory allowed specimens, samples from own body and specimens found in the Ilm park Weimar-Germany from water, plants and so on.

• Project idea 2: Can we implant Aliivibrio fischeri (bioluminescence bacteria) into Physarum Polycephalum (yellow microfungi)?

Wich kind of relationship could be made between the both of them? Is there any mutualism relationship? if there is, in which area can it be applied?

MICROORGANISM INVESTIGATION.

In order to decide which microorganism are suitable for the work that is going to be developed, an initial investigation is made in order to understand the characteristics of each microorganism, their needs in order to grow, and important information suitable for the project.

• List of bacterias and microorganism that can be cultivated in laboratories of high /medium security.

• • Bacillus Subtilis - (brown)
  • Chromobacterium violaceum - (violet)
  • Escherichia Coli - (colourless)
  • Micrococcus Luteus - (Yellow)
  • Micrococcus Roseus - (Rosa)
  • Proteus Mirabilis / Pseudomonas aeruginosa - (brown)
  • Pseudomonas Fluorescens – (Blue or green) fluorescens with pyoverdine
  • Serratia Marcescens – (Pink or Orange)
  • Staphylococcus aureus – (Yellow)
  • Vibrio Fischeri – (Bioluminescent)

• List of bacterias and microorganism that can be cultivated in laboratories of medium/basic security.

• • Polycephalum/physarum - (Yellow) tree form https://en.wikipedia.org/wiki/Physarum_polycephalum
  • Clorococcum - (green) https://en.wikipedia.org/wiki/Chlorococcum
  • Euglena gracilis - (green) https://en.wikipedia.org/wiki/Euglena_gracilis https://www.youtube.com/watch?v=UWSBcaJE6x4
  • Euglena Spirogyra / Lepocinclis Spirogyroides - (green/yellow)
  • Haematococcus Pluvialis - (red) https://en.wikipedia.org/wiki/Haematococcus_pluvialis https://www.youtube.com/watch?v=c6cCpyivi9E
  • Volvox Aureus - (granulated green) https://en.wikipedia.org/wiki/Volvox https://www.youtube.com/watch?v=lLvmHaz1uIw
  • Sternalge / Zygnema spec - (yellow/green) remoline form https://en.wikipedia.org/wiki/Zygnema
  • Blepharisma Americana - (red) https://www.youtube.com/watch?v=te_dW1vi6Ts
  • Blepharisma Japonicum - (red) https://en.wikipedia.org/wiki/Blepharisma_japonicum https://www.youtube.com/watch?v=5OB7kMCpDdM
  • Hidra Vulgaris - (white) long straight form https://www.youtube.com/watch?v=vkrxgNjmTsk
  • Alivibrio fischeri - (bioluminiscence) blue luminiscence https://en.wikipedia.org/wiki/Aliivibrio_fischeri

Here is a web page with information about some microorganism, how to cultivate them, the price of some of them. http://www.lebendkulturen.de/

MICROSCOPIC PHOTOGRAPHY.

The first step is to find a subject, in my case, I want to work with Polycephalum/physarum an allowed sample in the laboratory, with samples taking from the human body, could be like Escherichia Coli and also some other microorganism that can be found in the Ilm Park, in Weimar-Germany.

Types of photography with a microscope:

• Köhler Illumination: neutral and uniform backlighting, helps to maximize the contrast and resolution in the image.
  • https://www.zeiss.com/microscopy/int/solutions/reference/basic-microscopy/koehler-illumination.html
  • https://www.youtube.com/watch?v=dYP0uBxE0AA

• Darkfield: makes an object glow against a black background by using a darkfield condenser into the microscope (there are to types; dry or oil condenser).
  • https://en.wikipedia.org/wiki/Dark-field_microscopy
  • https://www.youtube.com/watch?v=Tw557w421is

Link to examples in microscopic photography & video work samples. https://www.youtube.com/watch?v=olCEGsKWQ3c

https://www.popphoto.com/tips-pro-microscopic-photography