GMU:Life in an aquatic ecosystem/Dahye Seo: Difference between revisions

Revision as of 16:00, 31 October 2024

Dead Life Live Life

* This project was started but not completed in SS 2023 and will be continued this semester.

Dead Life Live Life is a sound installation work that sonifies the movement of DNA. This work was inspired by the poetic movement of DNA in electrophoresis experiments. Dead Life Live Life explores the materiality of DNA itself rather than genetic analysis, which is the general purpose of DNA. “Dead life got the same material as live life”, biologist Lynn Margulis said in an interview. This could be paraphrased as “Dead life got the same DNA as live life”. Dead Life Live Life poetically represents the connection between life and death by sonifying the DNA movement of an old persimmon tree planted in my grandmother's house.

Technical solution

1. Biological part

Instruction of DNA analysis

1) DNA Extraction of Persimmons

2) PCR Experiment (DNA amplification) / Gel electrophoresis (DNA movement & visualization) DNA of the persimmon is amplified through a PCR experiment. The amplified DNA is visualized in a gel electrophoresis experiment. DNA molecules dyed to respond to UV light glow and flow electromagnetically in an agar-gel chamber.

2. Sonification part

1) Develop Max/Msp patch

Convert continuously changing number of RGB data (could be change to other data) of DNA movement animation to midi or frequency.

@@ Line 1: / Line 1: @@
-'''Project 1. Dead Life Live Life'''
+=== '''Dead Life Live Life''' ===
+<nowiki>*</nowiki> This project was started but not completed in SS 2023 and will be continued this semester.
-This project was started but not completed in SS 2023 and will be continued this semester.
+''Dead Life Live Life''  is a sound installation work that sonifies the movement of DNA. This work was inspired by the poetic movement of DNA in electrophoresis experiments. ''Dead Life Live Life''  explores the materiality of DNA itself rather than genetic analysis, which is the general purpose of DNA. “Dead life got the same material as live life”, biologist Lynn Margulis said in an interview.  This could be paraphrased as “Dead life got the same DNA as live life”. ''Dead Life Live Life''  poetically represents the connection between life and death by sonifying the DNA movement of an old persimmon tree planted in my grandmother's house.
-An overview of the work and its progress can be found below.
+[[File:Dlll 1.001.jpg|center|frameless|800x800px]]
+[[File:Dlll 2..jpg|center|frameless|800x800px]]
-[[GMU:Photosynthesis and I/Dahye Seo]]
+==== Technical solution ====
+===== 1. Biological part =====
+Instruction of DNA analysis<gallery>
+File:Instruction1 DNAworkshop.jpg
+File:Instruction2 DNAworkshop.jpg
+</gallery>1) DNA Extraction of Persimmons <gallery>
+File:Kaki gelElectrophoresis 2.jpeg
+File:Kaki gelElectrophoresis 3.jpeg
+File:Kaki gelElectrophoresis 4.jpeg
+File:Kaki gelElectrophoresis 5.jpeg
+File:Kaki gelElectrophoresis 6.jpeg
+File:Kaki gelElectrophoresis 7.jpeg
+File:Kaki gelElectrophoresis 8.jpeg
+</gallery>2) PCR  Experiment (DNA amplification) / Gel electrophoresis (DNA movement & visualization)
+DNA of the persimmon is amplified through a PCR experiment. The amplified DNA is visualized in a gel electrophoresis experiment. DNA molecules dyed to respond to UV light glow and flow electromagnetically in an agar-gel chamber. <gallery>
+File:Kaki gelElectrophoresis 10.jpeg
+File:Kaki gelElectrophoresis 9.jpeg
+File:Kaki gelElectrophoresis 11.jpeg
+File:Kaki gelElectrophoresis 12.jpeg
+File:Kaki gelElectrophoresis 13.jpeg
+File:Gelelectrophoresis workshop 20230428 Large.jpeg
+</gallery>
+===== 2. Sonification part =====
+) Develop Max/Msp patch
+Convert continuously changing number of RGB data (could be change to other data) of DNA movement animation to midi or frequency.{{#ev:youtube|bGzmhJ9kQi4}}