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== '''Fourth | == '''Fourth attempt'' == | ||
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Be careful with the disinfected working area. | Be careful with the disinfected working area. | ||
== Fifth attempt == | |||
After several times of experimenting with deferent types of nutrients and ways of applying the bacteria. I finally reach the expected result: | |||
'''''Experiment:'''' | |||
''Materials'' | |||
- 3 clean and disinfected petra dishes | |||
- 2 nutrient boiling containers | |||
- 1 pipet | |||
- 2 blades | |||
- 3 cotton swabs | |||
- 1 tweezers | |||
- 20 cubic centimeters foil | |||
The experiment will need 2 types of Agar. | |||
AGAR 1(100ml Nutrient): 100ml distilled water - 0,32g disinfectant - 2g Agar. | |||
AGAR 2 (100ml Nutrient): 100ml distilled water - 2g glucose - 0,5g peptone - 0,5g yeast extract - 0,27g Na2HPO4 - 2g Agar. | |||
ALL THE EXPERIMENT SHUND BE DEVELOP IN THE INFECTION FREE AREA¡ | |||
'''Step 1''' | |||
Cut the schemes on the foil and preserve in alcohol. | |||
'''Step 2''' | |||
Prepare AGAR 1, mix and heat until boiling. | |||
Put the AGAR 1 into the 3 disinfected petra dishes, add the schemes on the top, cover and wait until it draws. | |||
'''Step 3''' | |||
with the help of the blades and the tweezers cut the edges of the schemes in order to empty the design, cover and preserve | |||
'''Step 4''' | |||
Prepare AGAR 2, mix and heat until boiling. | |||
With the help of the pipet place the AGAR 2 into the empty places of the petra dishes, cover and preserve until it draws. | |||
'''Step 5''' | |||
Take bacteria from your skin with the cotton swabs and draw carefully into the AGAR 2 area, cover it and let it rest for about 8 days. | |||
== '''CLONING EXPERIMENT''' == | == '''CLONING EXPERIMENT''' == |
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