Marie EBEL (talk | contribs) No edit summary |
Marie EBEL (talk | contribs) No edit summary |
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[[File:STEP 3.9.JPG|400px]] | [[File:STEP 3.9.JPG|400px]] | ||
[[File:STEP 3.10.JPG|400px]] | [[File:STEP 3.10.JPG|400px]] | ||
=> I got no results : either the ladder nor the DNA samples were "growing" as they should (multiple lines). | |||
So I decided to test the ladder only to see if there is a problem from the samples themselves or with the preparation (agarose + buffer + distilled water) => #Second attempt | |||
#Second attempt | |||
[[File:STEP 3.2.1.jpg|400px]] | |||
[[File:STEP 3.2.2.jpg|400px]] | |||
[[File:STEP 3.2.3.jpg|400px]] | |||
[[File:STEP 3.2.4.jpg|400px]] | |||
=> Same problem : the ladder was growing as one bloc only (see the little drawing) + we noticed that the positive side was turning in blue color and some part of the agarose gel was melting down. | |||
So we decided to | |||
Revision as of 19:03, 17 January 2018
// HUMAN DNA ANALYSIS //
// PRACTICING PCR ANALYSIS //
Step 1. Extracting DNA from blood and from pork liver meat
Step 2. STR-analysis with pork meat primer
Step 3. Electrophoresis
- First attempt
=> I got no results : either the ladder nor the DNA samples were "growing" as they should (multiple lines). So I decided to test the ladder only to see if there is a problem from the samples themselves or with the preparation (agarose + buffer + distilled water) => #Second attempt
- Second attempt
=> Same problem : the ladder was growing as one bloc only (see the little drawing) + we noticed that the positive side was turning in blue color and some part of the agarose gel was melting down. So we decided to