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===Summary=== | ===Summary=== | ||
General introduction to working in the bio lab. Preparing the first testing '''Agar-Plates''' for bacterial growth in a petri dish: | General introduction to working in the bio lab. Preparing the first testing '''Agar-Plates''' for bacterial growth in a petri dish: | ||
*petri dishes where filled with 15 ml of an [[GMU:DIY Bio: doing things with biology/Introduction to conceptual framework and tools|LBP Medium]] with Agar Agar | *petri dishes where filled with 15 ml of an [[GMU:DIY Bio: doing things with biology/Introduction to conceptual framework and tools|LBP (Baird-Parker liquid) Medium]] with Agar Agar | ||
*cooling down | *cooling down | ||
*inoculateing with different surface smear | *inoculateing with different surface smear | ||
Revision as of 12:56, 22 October 2018
1st class: 16. Oktober 2018 - Introduction
Summary
General introduction to working in the bio lab. Preparing the first testing Agar-Plates for bacterial growth in a petri dish:
- petri dishes where filled with 15 ml of an LBP (Baird-Parker liquid) Medium with Agar Agar
- cooling down
- inoculateing with different surface smear
- In my case: human nose (inside), cat nose (outside) and fur, fridge glass surface and 1 cent coin
- left with room temperature for the following days
First culture medium
As described obove the filled petri dishes are no beeing observed for the following days.
Gallery
Day 1
Day 3
Day 6
Day 7
Breeding day 7 - closer look
Somehow I got the impression that the petri dishes weren't sterile as after 7 days almost the full dish is covered with a white film. You can only guess by slightly different colors where I initially inoculated the dish. Something seems to grow over the originally inoculated sections. See below some close ups. In the section of the example I took from my nose, a small yellow dot appears. I think this is what was intended to be growing. The rest just looks almost the same.
Gallery
Cat fur and outside nose
1 Euro cent
Day 7 - Full dish
Human nose inside
glass surface fridge
DIY Microscope Test
What was done?
- extracted converging lens from a laser pointer
- attached to iPhone SE back camera
Results
Chestetree seed
Sunflower seed
Sunflower and chestetree seed in comparison
Chestetree seed magnified
Sunflower seed magnified
- adding some filters to increase contrast und depth
Artsy chestetree seed
Artsy sunflower seed
