GMU:Https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY Bio: doing things with biology/Sirin Unmanee/30.10.2018 : Isolating Bacteria from Kombucha, Growing Physarum polycephalum (2nd attempt) and others&action=edit&redlink=1

Isolating Bacteria from Kombucha

Here is list of ingredients to prepare 500 ml of liquid Gluconcetobacter medium

Glucose - 10 g
Peptone - 2.5 g
Yeast extract - 2.5 g
Na2HPO4 - 1.35g
Citric acid - 0.75 g
Distilled or tap water - 500 ml
Agar - 7.5 g

However, in this case, I needed just 100 ml, so everything is divided by 5.

I dissolved 2 ml of Kombucha and 8 ml of distilled water, then smeared one drop of that solution onto the first slot. The others' solutions are dissolved in 1/2, 1/4, 1/8, 1/16 and 1/32 ratio respectively.

Growing Physarum polycephalum (2st attempt)

I failed growing slime mold, so I tried once again with three dishes (just in case!), but this time I keep all of them inside the cabinet in the lab. I've learnt form my 1st attempt that my room is not a suitable environment for growing the slime mold.

Growing Physarum polycephalum (2st attempt)