GMU:DIY Biolab License/Lea-Marie Weigelt

Project: Euglena

To-Do's

grow Euglena
care for Euglena
work out a creative Projekt using Euglena

Process of growing Euglena:

16th of November: start growing Euglena gracilis.

Making the substrate

For the substrate we followed this guide.

We decided to make 500ml of the solution so we could have a few tries from the beginning in case some of them failed. Working with live organisms is a lot of trial and error. For that I first did some calculations and multiplied all the necessary ingredient times five, since the recipe was originally for 100ml of solution. I wrote everything down to make it easier for myself once I actually got to weigh everything with the scales.

I got into the lab equipment which consists of a lab coat, gloves and goggles. I sterilised my hands before and after getting on the gloves, as well as my workspace and all my materials to minimize contaminations that could jeopardize growing the culture of Euglena.

I prepped my workspace with the necessary tools: a highly accurate scale (for the tiny amounts of chemicals you need), the necessary chemicals in order of the recipe, distilled water, a tiny spatula, tin foil as a base to put the chemicals on for weighing, my bottle for the finished solution; sanitizer and papertowels to clean the spatula between going into the different chemicals to prevent crosscontamination of products.

I made the base solution and solution one with the help of Alessandro. Solution 2 and 3 were kindly provided by Miga. In the base solution I then dropped the extra solutions 1 and 2 as specified in the text (5 times 8 drops for each) before they go to be autoclaved in the pressure cooker.

After preparing the solutions in their respective bottles we put them in the pressure cooker to kill off any spores or bacteria that might have landed in them while working (Make sure the lid of the bottle is on the bottle but not screwed all the way shut to prevent the glass from breaking in the pressure cooker. Also make sure somebody is there to watch the pressure cooker for workplace safety. Alessandro kindly did that for us while we went and got lunch).

Once everything was nice and sterile we prepared our workbench for making the actual substrate and put some Euglena in there for growing. We prepared all the solutions and put them where I can easily reach them. I also got the worksheet ready so I know how much has to go in the base solution, the vitamin solution and the Euglena sample to make sure I can work quickly and efficiently without having to search for things when I'm already working on it. Then I desinfected my hands, put gloves on and desinfect them again. Since we made more of the base solution I also had to take that into account when dropping in the other solutions. After the full substrate was done I filled four plastic containers until about 3/4 full and added a few of the drops with Euglena in them. Then we put all the tubes in a stand near a window to make sure the Euglena could photosynthesize and grow. Since it's winter Alessandro put up a UV lamp for them.

Footage of the grown Euglena

Problems/Difficulties that occured during the process

The first thing that happened that didn't turn out to be too big of a deal was that only one of the four tubes with Euglena that we made was actually usable. The others had contaminations and/or didn't grow. Which is a good reminder that these things can happen when you are working with living beings. So always have a backup and check in regularly to make sure they're still thriving.

Second thing was that I had a few problems with taking photos and videos from the microscope since I didn't really know how to make the camera take fluid and good quality videos. It was a little frustrating to look into the microscope and see so much color and life in the euglena only to see that the camera can't really pick up on these images as well as I'd hoped.

In general I noticed that one semester is way too short to grow organisms and also have enough time in the end to produce a satisfying art project with them. So I hope I'll get the opportunity to try again when there's more time and not a praxissemester coming up.

Documenation of the main Workshop 04.11.22

Plan for today (04.11) and tomorrow (05.11):

cultivate first organism (mushroom)
lab safety (how to work safely in the lab)
getting to know the lab equipment
Redesign the lab experience for our own home (How to make our own Diy-Biolab)
lab ethics and values

Materials and manual for growing first organisms:

Different organisms need different things to grow

examples:

Algae can photosynthesize; they need light and salt
slime mold (unicellar organisms that expand in search of food; they're neither fungi nor mushrooms)
bacteria

Building a Diy-Lab at home

Resources :

Equipment:

local shopping

hardware store (tables with a glass or ceramic top)
bricolage market
artists shop
pharmacies

online shopping

amazon; new equipment
Ebay; used equipment
Aliexpress; quick and cheap

consumables (cottonswabs/gloves etc.)

amazon; for small fast delivery
aliexpress; for cheap batch delivery
alibaba
specialised onlineshops; more or less specialised producers and retailers

chemicals

Buying at local stores (check cross-application; check purity grade according to use)
online shopping (check if law allows private purchase)
maybe extract your chemicals from mixtures (know what you are doing and with whom)

Growing our own mushrooms

For the Mycofabrication session we used Reishi (Ganoderma Lucidum)

what do we need?

1. Mushroom/spores

where can you find it:

online shops

Pilzmännchen.de
MycoGenetics

the forest:

on the ground
on dead logs or living trees

2. Substrates (mushroom food):

buy at local stores or online

Bricolage market (saw dust)
Pet shop
Carpenter

3. Knowledge

Books:

Mycelium running: how mushrooms can help save the world (2004)
Organic mushroom farming and mycoremediation (2014)
Radical Mycology: A Treatise on Seeing & working with Fungi (2016)
The fifth kingdom; An introduction to mycology Bryce Kendrick (2017)

Academical knowledge

first -> wikipedia and sources (gives good overview; sources; scientific papers)
second -> academic papers (google schola; unpaywall.org; if behind paywall: ask for copy; wikipedia.org/Sci-Hub: grey area of copy right)

"if you have a big goal break it up into small goals you can achieve"

Ethics and values in the lab

respect for our creatures
respect for lab partners
don't take criticism personally
only go to the lab when you are healthy and in the right mood (concentrated; healthy; good mindful mindstate)
respect time and projects of others (name everything; report when things run out or get broken)

How to respect our creatures and each other:

what is the specimen? (mushroom parts still thrive even if you take parts of it for example)
destroy things that aren't from here
nothing leaves the lab alive unless it comes from the natural habitat outside
we work to help understand more for everyone (goal: sustainability)
respect experts from other fields (everybody can provide value)
be inclusive; everybody is equal

05.11.22 10 am to 2 pm

Plan: Projectdraft

Presentation of some organisms

Brainstorming to one organism in groups of 3-4 people

what can we do with it?
how can we present that idea?
and create 5 ideas

Creatures:

slime mold: one big cell that grows toeards the food. What experiments can be done with it? Idea: how would slime mold plan rail networks
Euglena (Algae): can photosynthesize and reuse CO2 ; moves slowly towards the light
Mycelium: Idea: modular bricks for building structures
Glow in the dark mushrooms (pomellus stipticus): idea: exitlights/nightlights filled with mushrooms
Kombucha (yeast and bacteria living together) creating paperlike structure when dried

One hour to make up 5 ideas

Why? How? What?

1. Exit signs from glowing mushrooms (brightness?lifetime?)

   why? no, electricity but importance of finding the exit
   how? glowing mushroom jars 
   What? exit signs and emergency paths

2. Animation with Euglena (filming them moving towards the light; making templates that partly block out the light to create pictures etc.)

   why? for entertainment
   how? templates, light, experimentation
   what? animations, music videos, stop motion animation etc.

3. Lampshade from dried Combucha for interesting light effects

    why? warm light and interesting patterns 
    how? dry the combucha, make patterns for the lampshades 
    what? lampshades; windowshades

4. plates with slimemold layered to create interesting paintings

    why? new technique for artists; new medium
    how? slime mold grown on transparent plates, layered behind one another 
    what? layered paintings

5. Pens made from mycelium

   why? less plastic is used
   how? develop case for pens out of mycelium
   what? compostable pen cases; maybe Diy Kits for teens

Group session 2

Develop one of our ides further; chosen idea: glowing exit signs

structure:

why?

saving electricity
sustainability

realisation process

grow fungi (panellus stypticus)
inside glass cube
run some tests on when the mushroom glows
setup gives mushroom food and moisture
find out more about mushrooms biorythm

Plans for setup

reuse old exit-sign cases (test if it works with the plastic and color)
fill it with substrate and grow mushroom in there
install mushroom case

where do we want to exhibit it? Showcase 3 places here in Weimar

University
Lichthaus Kino
Jena botanischer Garten

Necessary tests:

biorythm testing
longtime study of mushroom lifespan
temperature? Is roomtemperature okay?
Nutrition? Is moisture in the substrate?
when does it glow? How does it decide to glow? Can it be used during the day?
examine exit signs; Are there brightnesslevels that have to be achieved? Is the case a suitable home for the mushroom? Who to ask for permission to install the mushroom cases