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==='''Tools''' === | ==='''Tools''' === | ||
====Microscopy==== | ====Microscopy==== | ||
[[File:Immersionsvorteil.jpg|thumb|"Principle of immersion microscopy. Path of rays with immersion medium (yellow) (left half) and without (right half). Rays (black) coming from the object (red) at a certain angle and going through the coverslip (orange, as the slide at the bottom) can enter the objective (dark blue) only when immersion is used."(https://en.wikipedia.org/wiki/Oil_immersion)]] | [[File:Immersionsvorteil.jpg|thumb|"Principle of immersion microscopy. Path of rays with immersion medium (yellow) (left half) and without (right half). Rays (black) coming from the object (red) at a certain angle and going through the coverslip (orange, as the slide at the bottom) can enter the objective (dark blue) only when immersion is used."(https://en.wikipedia.org/wiki/Oil_immersion)]] | ||
*[https://www.youtube.com/watch?v=SUo2fHZaZCU Basic Microscope Setup and Use] | *[https://www.youtube.com/watch?v=SUo2fHZaZCU Basic Microscope Setup and Use] | ||
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**''In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens. Immersion oils are transparent oils that have specific optical and viscosity characteristics necessary for use in microscopy.'' (https://en.wikipedia.org/wiki/Oil_immersion) | **''In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens. Immersion oils are transparent oils that have specific optical and viscosity characteristics necessary for use in microscopy.'' (https://en.wikipedia.org/wiki/Oil_immersion) | ||
*Further read and how to: http://www.micrographia.com/tutoria/micbasic/micbpt07/micb0700.htm | *Further read and how to: http://www.micrographia.com/tutoria/micbasic/micbpt07/micb0700.htm | ||
*''Microscopic Photography'' | *''Microscopic Photography'' | ||
The first step is to find a subject, in my case, I want to work with ''Polycephalum/physarum'' an allowed sample in the laboratory and with samples that will be brought by my colleagues. | The first step is to find a subject, in my case, I want to work with ''Polycephalum/physarum'' an allowed sample in the laboratory and with samples that will be brought by my colleagues. | ||
*''Types of photography with a microscope:'' | *''Types of photography with a microscope:'' | ||
**''Köhler Illumination:'' neutral and uniform backlighting, helps to maximize the contrast and resolution in the image. | **''Köhler Illumination:'' neutral and uniform backlighting, helps to maximize the contrast and resolution in the image. | ||
***https://www.zeiss.com/microscopy/int/solutions/reference/basic-microscopy/koehler-illumination.html | ***https://www.zeiss.com/microscopy/int/solutions/reference/basic-microscopy/koehler-illumination.html | ||
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*''DIY Microscopy'' | *''DIY Microscopy'' | ||
**[[GMU:DIY-Microscopy]] | **[[GMU:DIY-Microscopy]] | ||
**https://www.youtube.com/watch?v=9pQG9h8-nvQ | **https://www.youtube.com/watch?v=9pQG9h8-nvQ | ||