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The outcome of my project is an installation which makes it possible to move Euglena to places and in shapes I want. To | By working on my experiments I wondered how I can connect this idea with my previous work. I thought about building a connection between humans reaching for light and the organisms that do the same. | ||
Without thinking about the meaning I sometimes used a daylight lamp to light Euglena - its actual function is to spend light for people during the winter months to lift the spirits and to obviate tiredness. | |||
The outcome of my project is an Euglena painted picture which makes my thoughts visible, an installation which makes it possible to move Euglena to places and in shapes I want. To enable this I utilized Euglena’s ability of phototaxis to control its movement. The result is the copy of a negative drawing - filled with thousands of those green microorganisms - which starts to fade after a couple of minutes. | |||
Before I started to develop the installation I researched ideas, methods and other projects. The following experiments and the documentation shall give an overview of my work. | Before I started to develop the installation I researched ideas, methods and other projects. The following experiments and the documentation shall give an overview of my work. | ||
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<br> | |||
==='''50 Shades of Green - Euglena Experiment Kit'''=== | ==='''50 Shades of Green - Euglena Experiment Kit'''=== | ||
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<gallery> | <gallery> | ||
File: | File:Guide 1.png | ||
File: | File:Guide 2.png | ||
File:Guide 3.png | |||
File:Guide 4.png | |||
</gallery> | </gallery> | ||
- | ---- | ||
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== Research and Ideas == | == Research and Ideas == | ||
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*Is it possible to arrange Euglenas in forms I want them to be? | *Is it possible to arrange Euglenas in forms I want them to be? | ||
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<br> | |||
== Preparation == | == Preparation == | ||
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</gallery> | </gallery> | ||
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== Experiments == | == Experiments == | ||
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'''''How can I cultivate Euglena faster?''''' | '''''How can I cultivate Euglena faster?''''' | ||
For all the experiments I want to do I need a huge amount of | For all the experiments I want to do I need a huge amount of organisms. After observing the medium I already cooked about three weeks before without noticing any change, I wanted to try out something new. I researched some more advices and tips to speed up the reproduction. | ||
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'''Expactation''' | '''Expactation''' | ||
I guess that the nutrients I use this time work better for Euglena. During my researches I saw that various biology pages recommend the use of hard cheese and the addition of CO2. The tubes in which I am going to add CO2 will probably show a better growing of Euglena. Furthermore I think that in the tubes with the medium I have already made, the growing will be a bit faster because those | I guess that the nutrients I use this time work better for Euglena. During my researches I saw that various biology pages recommend the use of hard cheese and the addition of CO2. The tubes in which I am going to add CO2 will probably show a better growing of Euglena. Furthermore I think that in the tubes with the medium I have already made, the growing will be a bit faster because those Euglena already got some nutrients; the pure Euglena did not. | ||
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File:day 5.JPG | File:day 5.JPG | ||
</gallery> | </gallery> | ||
checking the vessels: | checking the vessels: | ||
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*Erlenmeyer flask - few amount of Euglena | *Erlenmeyer flask - few amount of Euglena | ||
*Beaker glass - amount of Euglena is significantly growing; only a few Paramecia | *Beaker glass - amount of Euglena is significantly growing; only a few Paramecia | ||
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[[File:tube 1 day 15.mov]] | [[File:tube 1 day 15.mov]] | ||
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*Erlenmeyer flask - moderate amount of Euglena; a few Paramecia | *Erlenmeyer flask - moderate amount of Euglena; a few Paramecia | ||
*Beaker glass - small amount of Euglena; a few Paramecia | *Beaker glass - small amount of Euglena; a few Paramecia | ||
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*Erlenmeyer flask - small amount of Euglena | *Erlenmeyer flask - small amount of Euglena | ||
*Beaker glass - small amount of Euglena | *Beaker glass - small amount of Euglena | ||
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*Erlenmeyer flask - cheese on the bottom starts becoming green; moderate amount of Euglena | *Erlenmeyer flask - cheese on the bottom starts becoming green; moderate amount of Euglena | ||
*Beaker glass - cheese on the bottom starts becoming green; small amount of Euglena | *Beaker glass - cheese on the bottom starts becoming green; small amount of Euglena | ||
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File:after shaking.JPG | File:after shaking.JPG | ||
</gallery> | </gallery> | ||
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*Erlenmeyer flask - moderate amount of Euglena; fast movement | *Erlenmeyer flask - moderate amount of Euglena; fast movement | ||
*Beaker glass - moderate amount of Euglena; fast movement | *Beaker glass - moderate amount of Euglena; fast movement | ||
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*Erlenmeyer flask - high amount of Euglena; fast movement | *Erlenmeyer flask - high amount of Euglena; fast movement | ||
*Beaker glass - moderate amount of Euglena; fast movement | *Beaker glass - moderate amount of Euglena; fast movement | ||
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*Erlenmeyer flask - high amount of Euglena; fast movement | *Erlenmeyer flask - high amount of Euglena; fast movement | ||
*Beaker glass - moderate amount of Euglena; fast movement | *Beaker glass - moderate amount of Euglena; fast movement | ||
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*Erlenmeyer flask - high amount of Euglena; fast movement | *Erlenmeyer flask - high amount of Euglena; fast movement | ||
*Beaker glass - high amount of Euglena; fast movement | *Beaker glass - high amount of Euglena; fast movement | ||
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<br> | <br> | ||
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</gallery> | </gallery> | ||
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==== Experiment 3 ==== | ==== Experiment 3 ==== | ||
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[[File:DSC03183.JPG|400px]] | [[File:DSC03183.JPG|400px]] | ||
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<br> | <br> | ||
== Developing an Euglena Experiment Kit == | == Developing an Euglena Experiment Kit == | ||
<br> | |||
Experimenting with Euglena in different ways made me even more interested in this organism. Especially the experiments with the shapes were very exciting and fascinating. So I decided to develop a toolkit (or the prototype of a toolkit) which makes it easy to arrange similar projects. I still have more ideas what I could do with the tools I have and I am curious what kind of ideas other people have regarding this topic. | |||
Experimenting with Euglena in different ways made me even more | |||
''Remark:'' I developed this experiment kit based on the knowledge of Euglena I have by now. The main focus on this project is giving interested people the chance to experiment with Euglena artistically on a low budget. | ''Remark:'' I developed this experiment kit based on the knowledge of Euglena I have by now. The main focus on this project is giving interested people the chance to experiment with Euglena artistically on a low budget. | ||
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'''What can you do with it?''' The installation is made for experimenting with Euglena in connection with light, colors and shapes. People who are interested can use it for playing around and turn their pictures into a very different form of drawing. I think it is especially interesting because you can control the development of the outcome not completely. At some point you need to give up the control over your artwork and let Euglena surprise you. You can draw your very own picture, convert it into a mask and receive an Euglena based drawing as outcome. By taking a photo before the artwork fades, you can create a visual memory which can also be printed (e.g. on linen) as art print. | '''What can you do with it?''' The installation is made for experimenting with Euglena in connection with light, colors and shapes. People who are interested can use it for playing around and turn their pictures into a very different form of drawing. I think it is especially interesting because you can control the development of the outcome not completely. At some point you need to give up the control over your artwork and let Euglena surprise you. You can draw your very own picture, convert it into a mask and receive an Euglena based drawing as outcome. By taking a photo before the artwork fades, you can create a visual memory which can also be printed (e.g. on linen) as art print. | ||
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<br> | |||
==Conclusion== | |||
===Cultivating Euglena=== | |||
'''After nearly one month of cultivating Euglena the color of all cultures is finally green. Time to sum up the results.''' | '''After nearly one month of cultivating Euglena the color of all cultures is finally green. Time to sum up the results.''' | ||
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'''My recommendation for growing Euglena:''' | '''My recommendation for growing Euglena:''' | ||
Miga’s recipe | Miga’s recipe in combination with a small piece of hard cheese, a warm and light place but no direct sunlight, at least three weeks time and some patience :) | ||
''Note:'' I don’t think the extra solution 3 of the medium is needed anymore because the cheese contains Vitamin B12. To substitute Vitamin B1 some wheat grain should be added as well. I often read that this I used for breeding Euglena too, for example [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/images/Culturing-Euglena2.pdf here]. | ''Note:'' I don’t think the extra solution 3 of the medium is needed anymore because the cheese contains Vitamin B12. To substitute Vitamin B1 some wheat grain should be added as well. I often read that this I used for breeding Euglena too, for example [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/images/Culturing-Euglena2.pdf here]. | ||
===Working artistically with Euglena=== | |||
Sharing a room with Euglena for a whole semester gave me a lot of inspiration - how this organism works, how simple and difficult is is at once and what methods I can use to build a bridge between our livings. | |||
The fact that Euglena is able to do photosynthesis opens so many doors which can be used to find artistic ways to work with microorganisms. | |||
As someone who never did something like this before I am very excited about the process and the outcome. Even though I struggled at the beginning with breeding Euglena I found my workflow and finally managed to develop an idea and to be happy with the result. |
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