Fomes fomentarius (Tinder Mushroom): Difference between revisions

Fomes fomentarius (Tinder Mushroom) (view source)

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 <u>Step 1: Weigh the Substrate</u>
+* Combine the solid components in a bowl and weigh them.
+* Add distilled water to the mixture.
+* Mix thoroughly, ensuring to break down any chunks and evenly distribute the moisture throughout the substrate.
+* Transfer the substrate into a filter bag or an autoclavable container equipped with an air filter.
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 <u>Step 2: Sterilise</u>
+* Sterilize the bag by pressure cooking it for 50 minutes.
+* After sterilization, allow the bag to cool down. Note that the substrate can retain heat for several hours, so always squeeze it gently to assess the temperature at the center.
 <u>Step 3: Mix In Mycelium</u>
+* If using grain spawn, break it down by shaking the jar or by using a sterile or flamed spoon to mix the grains.
+* If the liquid culture is not already fragmented, mix the contents well with a sterile or flamed spoon.
+* Pour or spread the inoculant into the bag and seal it.
+* Roll the bag to thoroughly mix the contents.
 <u>Step 4: Incubate</u>
+* Incubate in darkness for 1 to 6 weeks.
+* Check the progress of the mycelium every two to three days, and monitor for any signs of contamination.
 For reference to the original experiment, please see [https://www.top-ev.de/wp-content/uploads/2021/12/Prepare-small-mycelium-bags-v.2.0-1.pdf Preparation of a Small Bag with Substrate]