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##To grow them in the laboratory## | |||
we needed to reproduce their living environment and feed them. | |||
We cultivated the specie ''Hypsibius exemplaris'' in a 25ml plastic bottle. | We cultivated the specie ''Hypsibius exemplaris'' in a 25ml plastic bottle. | ||
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Once a week, we fed the algae to the tardigrades as follows (each step needs a different sterile pipette tip): | Once a week, we fed the algae to the tardigrades as follows (each step needs a different sterile pipette tip): | ||
* Remove from the tardigrade culture half of the water on the top, without moving the biomass on the bottom | |||
* Replace the taken amount of water with the water coming from the Chlorococcus culture, aiming at taking the algae. | |||
* Refill the Chlorococcus culture with sterile cultivation medium (spring water with 0,01% of fertilizer algabloom) | |||
To observe them, with a sterile tip, take 200µl of water in the biomass at the bottom, put them on a slide and use the 10x magnification lens of the microscope to see them. [[File:In the lab.png|thumb|disolving the sample]] | To observe them, with a sterile tip, take 200µl of water in the biomass at the bottom, put them on a slide and use the 10x magnification lens of the microscope to see them. [[File:In the lab.png|thumb|disolving the sample]] | ||