GMU:Life in an aquatic ecosystem/Dahye Seo: Difference between revisions

GMU:Life in an aquatic ecosystem/Dahye Seo (view source)

310 bytes added , 16 November

217

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 .11.16. Sat, 9:30 am
-Gel Electrophoresis Exercise
+<u>Gel Electrophoresis Exercise</u>
-How to use micro pipette : <nowiki>https://youtu.be/uNzQqCW7ZH8?si=pcJW3g94jrHcv8ao</nowiki>
+I started by relearning how to use a micropipette to pick up where I left off a year ago. I tested two DNA ladders and a green stain that I had stored in the freezer to see if they were still functional.
-Gel electrophoresis Experiment : <nowiki>https://youtu.be/nZpA8IoMub0?si=OPH9O0mFwgGco-CV</nowiki>
-<gallery>
+Total 3 electrophoresis (see photos below)
+V - 40 min -> 5V - 40 min -> 6V - 60 min
+The instructions say to electrophoresis at 5v for 40 min. Due to the short distance of DNA migration, I did 2 additional cycles.
+Result<gallery>
 File:First round 5V-40min.jpg
 File:2round 5v-40min.jpg
 File:3round 6v-60min.jpg
-</gallery>
+</gallery>*DNA moves slow. Next time, reduce the percentage of agarose gel.