167
edits
GMU:Life in an aquatic ecosystem/Dahye Seo (view source)
Revision as of 21:37, 16 November 2024
, Saturday at 21:37no edit summary
No edit summary Tag: 2017 source edit |
No edit summary |
||
| Line 49: | Line 49: | ||
2024.11.16. Sat, 9:30 am | 2024.11.16. Sat, 9:30 am | ||
<u> | <u>Introduction</u> | ||
Gel Electrophoresis Exercise | |||
I started by relearning how to use a micropipette to pick up where I left off a year ago. I tested two DNA ladders and a green stain that I had stored in the freezer to see if they were still functional. | I started by relearning how to use a micropipette to pick up where I left off a year ago. I tested two DNA ladders and a green stain that I had stored in the freezer to see if they were still functional. | ||
<u>Procedure</u> | |||
The instructions say to electrophoresis at 5v for 40 min. Due to the short distance of DNA migration, I did 2 additional cycles. | The instructions say to electrophoresis at 5v for 40 min. Due to the short distance of DNA migration, I did 2 additional cycles.Running Gel electrophoresis 3 times | ||
5V - 40 min -> 5V - 40 min -> 6V - 60 min | 5V - 40 min -> 5V - 40 min -> 6V - 60 min | ||
| Line 70: | Line 72: | ||
Result<gallery> | |||
Result | |||
First Column: 1 kb DNA ladder | |||
Last to first and second Columns: 100 bp DNA ladder <gallery> | |||
File:First round 5V-40min.jpg | File:First round 5V-40min.jpg | ||
File:2round 5v-40min.jpg | File:2round 5v-40min.jpg | ||