GMU:Life in an aquatic ecosystem/Dahye Seo: Difference between revisions

GMU:Life in an aquatic ecosystem/Dahye Seo (view source)

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 <u>Procedure</u>
-The instructions say to electrophoresis at 5v for 40 min. Due to the short distance of DNA migration, I did 2 additional cycles.Running Gel electrophoresis 3 times
+''recipe''
-V - 40 min -> 5V - 40 min -> 6V - 60 min
+Agarosegel : 0.5g Agarose, 50 ml TBE Buffer, 2.5 µl DNA Stein green
+TBE Buffer for cover gel : 50 ml TBE Buffer, 1.25 µl DNA Stein green
+Gel Electrophoresis  : 5V - 40 min -> 5V - 40 min -> 6V - 60 min
+The instructions say to electrophoresis at 5v for 40 min. Due to the short distance of DNA migration, I did 2 additional cycles.
 <gallery>