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[[File:PCR_Side view_wiki.jpg]] | [[File:PCR_Side view_wiki.jpg]] | ||
A finished PRC Machine : one of three | |||
With the 3 PCR's assembled and ready on Friday evening, Saturday morning came where we followed a DNA extraction protocol using the solutions (Primers.. Master mix.. Buffers.ec.t..) which came from a PRC Kit. | With the 3 PCR's assembled and ready on Friday evening, Saturday morning came where we followed a DNA extraction protocol using the solutions (Primers.. Master mix.. Buffers.ec.t..) which came from a PRC Kit. | ||
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[[File:PCR_cycles_running.jpg]] | [[File:PCR_cycles_running.jpg]] | ||
PCR Starts its first cycle! | The PCR Starts its first cycle! | ||
1. 95 °C Denaturation step. First, you heat the DNA to a high temperature (95 °C) so that the two strands of genomic DNA, and later PCR DNA, separate. | 1. 95 °C Denaturation step. First, you heat the DNA to a high temperature (95 °C) so that the two strands of genomic DNA, and later PCR DNA, separate. | ||
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3. Extension Step. Third, you raise the temperature to about 70 °C to activate a DNA polymerase and elongate the primer with respect to the template strand. | 3. Extension Step. Third, you raise the temperature to about 70 °C to activate a DNA polymerase and elongate the primer with respect to the template strand. | ||
This sequence above is repeated about 35 times. This results in 34,359,738,368 copies of the starting DNA sample. | This sequence above is repeated about 35 times. This results in 34,359,738,368 copies of the starting DNA sample. | ||
After the first three cycles, we will have the first perfect copy. | |||
POST PCR... | |||
There are several Methods for analysing DNA. Here we are making a gel Chamber for electrophoresis. | |||
This basically is running an a electrical current though some gel. One positive cathode at one end, and the the Negative at the other. | |||
By setting the gel with a 'comb' you create small pockets where the DNA samples can be added along with a tracer. | |||
[[File:Combs_gel_Chamber.jpg]] | |||
The combs in place, ready for the gel to be added.. | |||
[[File:Filling_Gel-Chamber.jpg]] | |||
Filling the Chamber with gel. Then leaving it to set, before adding the Traces with Amplified DNA. | |||
My Phone died at this point and some I have no more footage. | |||