133
edits
No edit summary |
mNo edit summary |
||
Line 23: | Line 23: | ||
Think no further than ''the soil''. | Think no further than ''the soil''. | ||
''Streptomyces''. | |||
---- | |||
===ISOLATION OF STREPTOMYCES=== | ===ISOLATION OF STREPTOMYCES=== | ||
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment==== | ====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment==== | ||
Line 50: | Line 50: | ||
====6th step: Incubate at 30° for 5-7 days==== | ====6th step: Incubate at 30° for 5-7 days==== | ||
[[File:Incubation.png|250px]] | |||
::Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria | ::Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria | ||
---- | ---- |
edits