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(Created page with "== Microscopy with Oil Immersion and inspection of previous samples== "In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope....") |
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"In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens. Immersion oils are transparent oils that have specific optical and viscosity characteristics necessary for use in microscopy."(https://en.wikipedia.org/wiki/Oil_immersion) | "In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens. Immersion oils are transparent oils that have specific optical and viscosity characteristics necessary for use in microscopy."(https://en.wikipedia.org/wiki/Oil_immersion) | ||
[[File:Immersionsvorteil.jpg|600px]] | [[File:Immersionsvorteil.jpg|600px|"Principle of immersion microscopy. Path of rays with immersion medium (yellow) (left half) and without (right half). Rays (black) coming from the object (red) at a certain angle and going through the coverslip (orange, as the slide at the bottom) can enter the objective (dark blue) only when immersion is used."(https://en.wikipedia.org/wiki/Oil_immersion)]] | ||
Further read and how to: http://www.micrographia.com/tutoria/micbasic/micbpt07/micb0700.htm | Further read and how to: http://www.micrographia.com/tutoria/micbasic/micbpt07/micb0700.htm |