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Freya Probst (talk | contribs) |
Freya Probst (talk | contribs) |
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: Under the right conditions one Bacteria can divide itself infinetely and pile up a colony of its kind. The procedure of serial dilution (reducing the amount of bacteria in a solution within steps) is for example useful for counting bacteria. The Gluconacetobacter that we extracted out of the Kombucha Tea also changed its colonies with different concentration of bacteria in a solution (distilled water). | : Under the right conditions one Bacteria can divide itself infinetely and pile up a colony of its kind. The procedure of serial dilution (reducing the amount of bacteria in a solution within steps) is for example useful for counting bacteria. The Gluconacetobacter that we extracted out of the Kombucha Tea also changed its colonies with different concentration of bacteria in a solution (distilled water). | ||
'''Medium for the Gluconacetobacter:''' | '''1. Medium for the Gluconacetobacter:''' | ||
10 g Glucose | 10 g Glucose | ||
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7.5 g Agar | 7.5 g Agar | ||
'''2. Dilution Procedure''' | |||
:We had 6 Petridishes that contained our medium and our pipette could collect 3 ml of liquid. As a first step, we blended a piece of the Kombucha skin with 0.5 ml of distilled water and poured a drop onto our first dish. The first 0.5 ml of bacteria-solution was diluted further with additional 2.5 ml of distilled water. Another drop was poured onto the second dish. We emptied the pipette again till we regained the amount of 0.5 ml and added another 2.5 ml of distilled water. | :We had 6 Petridishes that contained our medium and our pipette could collect 3 ml of liquid. As a first step, we blended a piece of the Kombucha skin with 0.5 ml of distilled water and poured a drop onto our first dish. The first 0.5 ml of bacteria-solution was diluted further with additional 2.5 ml of distilled water. Another drop was poured onto the second dish. We emptied the pipette again till we regained the amount of 0.5 ml and added another 2.5 ml of distilled water. | ||
'''3. Outcome''' | |||
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We could observe how the Gluconacetobacter colonies arranged differently according to its concentration on the petridish. | :We could observe how the Gluconacetobacter colonies arranged differently according to its concentration on the petridish. |
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