Bureaucrats, emailconfirmed, Administrators
5,468
edits
GMU:Board Games for Humans and Nonhumans/Photosynthetic Organisms (view source)
Revision as of 10:44, 4 May 2017
, 4 May 2017→Euglena
| Line 65: | Line 65: | ||
“Most species of Euglena have photosynthesizing chloroplasts within the body of the cell, which enable them to feed by autotrophy, like plants. However, they can also take nourishment heterotrophically, like animals.” (wikipedia) | “Most species of Euglena have photosynthesizing chloroplasts within the body of the cell, which enable them to feed by autotrophy, like plants. However, they can also take nourishment heterotrophically, like animals.” (wikipedia) | ||
====Medium==== | |||
"Combine 1 L of spring water or Chalkley’s 1x solution or Pringsheim’s, 20 wheat or rice grains, and 5 mL (1 tsp) of dry | |||
powdered milk in a beaker. Boil the mixture for 5–10 minutes, and then dilute this mixture with 3 L of spring water. Let this | |||
solution cool and stand uncovered for 24 hours. | |||
Shake or stir the media solution and fill shallow containers with wide bottoms approximately half full. (Stacking culture bowls work best.) Add 50–100 mL of Euglena stock culture to each bowl. The Euglena culture medium contains a great deal | |||
of organic matter. Bacteria will grow rapidly and the cultures are likely to have a pungent odor for several days. (Take this into | |||
consideration when selecting a culture storage location.) | |||
Chalkley’s Stock Solution 10x* | |||
0.06 g CaCl2 | |||
1.00 g NaCl | |||
0.04 g KCl | |||
1 L Distilled water | |||