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GMU:DIY Biolab “Driver’s License”/Marie Laure Ebel (view source)
Revision as of 19:26, 17 January 2018
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[[File:STEP 3.2.4.jpg|400px]] | [[File:STEP 3.2.4.jpg|400px]] | ||
=> Same problem : the ladder was growing as one bloc only (see the little drawing) | => Same problem : the ladder was growing as one bloc only (see the little drawing). Even during a 2.2 attempt with new SERVA Stain G (which I thought it could be the problem, since the previous tube was quiet old). Then, I noticed that the positive side was turning in blue color and some part of the agarose gel was melting down. | ||
So | So I decided to test if this bleu color is coming from oxidation of the electrodes themselves, or if the ladder was "swimming" on the extra amount of solution (buffer TAE 50x + distilled water) above the agarose to reach the positive side => #Third attempt | ||
#Third attempt | #Third attempt | ||
[[File:STEP 3.3.1.jpg|400px]] | |||
[[File:STEP 3.3.2.jpg|400px]] | |||
=> Still turning bleu. Even with smaller electrodes that are completely covered by the solution (buffer TAE 50x + distilled water) | |||
So I decided to test if this oxidation is coming from the buffer or if it is simply a natural process => #Fourth attempt | |||
#Fourth attempt | |||
[[File:STEP 3.4.1.jpg|400px]] | |||
[[File:STEP 3.4.2.jpg|400px]] | |||
=> First picture : distilled water only. Second picture : distilled water + buffer TAE 50x. No changes at all. | |||
So it might come from the SERVA Stain G (which is yellow-ish) or it is actually not a problem and does not affect on the electrophoresis itself. | |||