385
edits
GMU:DIY Biolab “Driver’s License”/Marie Laure Ebel (view source)
Revision as of 17:37, 5 February 2018
, 5 February 2018→#Second attempt
Marie EBEL (talk | contribs) |
Marie EBEL (talk | contribs) |
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====#Second attempt==== | ====#Second attempt==== | ||
[[File:STEP 3.2.1.jpg|170px]] | |||
File:STEP 3.2.1.jpg| | [[File:STEP 3.2.2.jpg|170px]] | ||
File:STEP 3.2.2.jpg| | [[File:STEP 3.2.3.jpg|170px]] | ||
File:STEP 3.2.3.jpg| | [[File:STEP 3.2.4.jpg|170px]] | ||
File:STEP 3.2.4.jpg| | |||
=> Same problem: the ladder was growing as one bloc only (see the little drawing). Even during a 2.2 attempt with new SERVA Stain G (which I thought it could be the problem since the previous tube was quite old). Then, I noticed that the positive side was turning in blue color and some part of the agarose gel was melting down. | => Same problem: the ladder was growing as one bloc only (see the little drawing). Even during a 2.2 attempt with new SERVA Stain G (which I thought it could be the problem since the previous tube was quite old). Then, I noticed that the positive side was turning in blue color and some part of the agarose gel was melting down. | ||
So I decided to test if this blue color is coming from oxidation of the electrodes themselves, or if the ladder was "swimming" on the extra amount of solution (buffer TAE 50x + distilled water) above the agarose to reach the positive side => #Third attempt | So I decided to test if this blue color is coming from oxidation of the electrodes themselves, or if the ladder was "swimming" on the extra amount of solution (buffer TAE 50x + distilled water) above the agarose to reach the positive side => #Third attempt | ||
====#Third attempt==== | ====#Third attempt==== | ||