GMU:DIY Biolab “Driver’s License”/Suna Yoo: Difference between revisions

So i took put on my new rollerskates (3) and desinfected the whole kitchen with Korn (4), a typical thuringian alcohol. Then I boiled the coffee mold (5) for 15 minutes, maybe longer and put it in a bowl to let it cool down (6). Then i desinfected an old paprika sauce glass with Korn and put the boiled mold inside (7) rotatory with pieces of the dropped spores (8&9-11) and tapped it with kitchen paper. In the end i heated my oven to 50 degrees, let it cool down and then stored the glass in it with circulating air (12). Later that day i went to the lab to put in in the real machine.

Only the sequence between the beginning and end primer is being redoubled, that makes diffrent DNA´s comparable. At least parts of it. I think Julian brought us the primer that is used to seperate the part of the Helix that stores the information about our regional origin.

   

7. Day. Today we wanted to use gel electrophoresis to compare and analize our DNA, but it didn´t work. We don´t know why.

I didn´t want to throw away the rest of the culture, so i just cut out the contamination, to see if the mycelium survives or can take back the control.

10. Day. That Day I went to the Lab to make my own PDA-medium. To experiment more with mycelium and find out wich are the best conditions to grow it. Because i think for the paper production i need a lot more mycelium, i needs to be well moisture, for a stable growth, but nor to much medium, so that it can eat up the whole of it and i don´t have to dissolve the medium from the mycelium, because the water is not good for the mycelium either.  

Therefore i cooked some old potatoes:

and boiled them for maybe 20 minutes, than took them out of the water:

I waited:

And cut out mycelium from my last experiment with a knife, disinfected with fire. With those mycelium pieces, i inoculated the PDA-medium filled petridishes. I wanted to do each in a different way, to see how i can achive the best result to produce paper from it. Therefore i varied the amount of medium and sometimes put the mycelium upside down in the medium. Additionally i wanted to know if the mycelium maybe grows better in the closet than in the oven. So i put of every kind one in the oven and one in the closet to make it comparable and marked it as you can see in the gallery below: